Production of antimicrobial films by incorporation of partially purified lysozyme into biodegradable films of crude exopolysaccharides obtained from Aureobasidium pullulans fermentation
Küçük Resim Yok
Tarih
2005
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Faculty Food Technology Biotechnology
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Antimicrobial films were produced by incorporating partially purified lysozyme into films of crude exopolysaccharides (59 % pullulan) obtained from Aureobasidium pullulans fermentation. After film making, the films containing lysozyme at 100, 260, 520 and 780 mu g/cm(2) showed 23 to 70 % of their expected enzyme activities. The highest recovery of enzyme activity (65-70 %) after the film making was obtained in films prepared by incorporating lysozyme at 260 mu g/cm(2) (1409 U/cm(2)). The incorporation of disodium EDTA(.)2H(2)O and sucrose did not affect the initial lysozyme activity of the films significantly. With or without the presence of disodium EDTA(.)2H(2)O at 52 or 520 mu g/cm(2), lysozyme activity showed sufficient stability in the films during 21 days of cold storage. However, the presence of sucrose at 10 mg/cm(2) in the films caused the destabilization of part of enzyme activity (almost 35 %) at the end of storage. The combinational incorporation of lysozyme at 780 pg/cm(2) (4227 U/cm(2)) and disodium EDTA(.)2H(2)O at 520 mu g/cm(2) gave antimicrobial films effective on Escherichia coli. However, in the studied lysozyme concentration range the films did not show any antimicrobial activity against Lactobacillus plantarum. This study clearly showed that the partially purified lysozyme and crude exopolysaccharides from Aureobasidium pullulans may be used to obtain antimicrobial films to increase the safety of foods.
Açıklama
Anahtar Kelimeler
lysozyme, exopolysaccharides, pullulan, Aureobasidium pullulans, antimicrobial films
Kaynak
Food Technology and Biotechnology
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
43
Sayı
4
