A highly sensitive DNA aptamer-based fluorescence assay for sarcosine detection down to picomolar levels

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dc.contributor.authorOzyurt, Canan
dc.contributor.authorCanbay, Zeynep Celik
dc.contributor.authorDinckaya, Erhan
dc.contributor.authorEvran, Serap
dc.date.accessioned2019-10-27T09:44:19Z
dc.date.available2019-10-27T09:44:19Z
dc.date.issued2019
dc.departmentEge Üniversitesien_US
dc.description.abstractSarcosine is an amino acid derivative, which is considered as a key metabolite in various metabolic processes. Therefore, simple and sensitive detection methods are needed for further understanding its metabolic role and diagnostic value. In this study, we developed a novel method that meets the need for practical and sensitive detection in a complex medium mimicking urine conditions. For this aim, we selected sarcosine-specific DNA aptamers using graphene oxide-assisted systemic evolution of ligands by exponential enrichment (GO-SELEX). The candidate aptamers were labeled with 6-carboxyfluorescein (6-FAM) at their 5' ends. Two aptamers, namely 9S and 13S produced a significant fluorescence signal upon sarcosine binding. Both aptamers enabled a sensitive analysis with a detection limit of 0.5 pM. The linear detection ranged between 5 pM and 50 mu M for 9S aptamer, while 13S aptamer enabled a wider linear detection range between 5 pM and 500 mu M. The aptamer-based assay allowed rapid detection with no need for chemical derivatization of sarcosine and sophisticated instruments. Moreover, the aptamer-based assay was free of interference from urea and human serum albumin. (C) 2019 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [215Z182]en_US
dc.description.sponsorshipWe acknowledge the financial support of the Scientific and Technological Research Council of Turkey (TUBITAK) (grant number 215Z182).en_US
dc.identifier.doi10.1016/j.ijbiomac.2019.02.030en_US
dc.identifier.endpage97en_US
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.pmid30738156en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage91en_US
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2019.02.030
dc.identifier.urihttps://hdl.handle.net/11454/28985
dc.identifier.volume129en_US
dc.identifier.wosWOS:000466621200011en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofInternational Journal of Biological Macromoleculesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDNA aptameren_US
dc.subjectGO-SELEXen_US
dc.subjectSarcosineen_US
dc.titleA highly sensitive DNA aptamer-based fluorescence assay for sarcosine detection down to picomolar levelsen_US
dc.typeArticleen_US

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