Comparison of Aneuploidy Frequency to Sperm FISH and Sperm Apoptosis Results in Embryos That Lost the Vitality

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dc.contributor.authorCankaya, Tufan
dc.contributor.authorÖzkınay, Cihangir
dc.contributor.authorGunduz, Cumhur
dc.contributor.authorTavmergen, Erol
dc.contributor.authorBora, Elcin
dc.contributor.authorÖzkınay, Ferda
dc.date.accessioned2019-10-27T21:37:05Z
dc.date.available2019-10-27T21:37:05Z
dc.date.issued2011
dc.departmentEge Üniversitesien_US
dc.description.abstractObjective: Rate of chromosomal abnormality which is seen in embryos is higher than those in spontaneous aborts. This rate varies from 23 to 83 percent. In this study; in embryos with development arrest, we aimed To investigate the number abnormalities in 13(rd), 16(th), 18(th), 21(st) and 22(nd) chromosomes, To show the correlation between the chromosomal abnormality rates in embryos with developmental arrest and rate of live embryos in same cycle. To investigate the correlation between the apoptosis and the chromosomal number abnormalities in embryos with developmental arrest and sperm cells that are obtained in same cycle. Material and Methods: In twenty embryos with developmental arrest, the number abnormalities of 13(rd), 16(th), 18(th), 21(st) and 22(nd) chromosomes were analyzed with FISH method. The chromosomal numbers of X, Y and 18(th) chromosomes were analyzed with same methods in sperm cells that are obtained from the fathers of embryos at the same cycle. Apoptosis status in sperm cells was assessed with TUNNEL test. Results: The chromosomal number abnormality ratewas 80% in embryos with developmental arrest. No significant correlation was detected between the abnormality rate in embryos and living embryo rate as well as total abnormality rate in sperm cells. No correlation was detected between sperm apoptosis and chromosomal abnormality rates in embryos. However a significant correlation was detected between chromosomal abnormality rate and spermiogram results. Conclusion: In embryos with developmental arrest, chromosomal abnormality rates were higher in 13(rd), 16(th), 18(th), 21(st) and 22(nd) chromosomes. Chromosomal abnormality and apoptosis frequency in sperms were not significantly correlated with chromosomal abnormalities in embryos however a significant correlation was found between spermiogram results of fathers and chromosomal abnormalities in embryos with developmental arrest.en_US
dc.identifier.doi10.5336/medsci.2010-16982
dc.identifier.endpage903en_US
dc.identifier.issn1300-0292
dc.identifier.issn2146-9040
dc.identifier.issn1300-0292en_US
dc.identifier.issn2146-9040en_US
dc.identifier.issue4en_US
dc.identifier.startpage896en_US
dc.identifier.urihttps://doi.org/10.5336/medsci.2010-16982
dc.identifier.urihttps://hdl.handle.net/11454/46165
dc.identifier.volume31en_US
dc.identifier.wosWOS:000296212300021en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isotren_US
dc.publisherOrtadogu Ad Pres & Publ Coen_US
dc.relation.ispartofTurkiye Klinikleri Tip Bilimleri Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPreimplantation diagnosisen_US
dc.subjectin situ hybridizationen_US
dc.subjectfluorescenceen_US
dc.subjectblastomeresen_US
dc.subjectaneuploidyen_US
dc.subjectin situ nick-end labelingen_US
dc.titleComparison of Aneuploidy Frequency to Sperm FISH and Sperm Apoptosis Results in Embryos That Lost the Vitalityen_US
dc.typeArticleen_US

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