Comparison of Aneuploidy Frequency to Sperm FISH and Sperm Apoptosis Results in Embryos That Lost the Vitality
Küçük Resim Yok
Tarih
2011
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Ortadogu Ad Pres & Publ Co
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective: Rate of chromosomal abnormality which is seen in embryos is higher than those in spontaneous aborts. This rate varies from 23 to 83 percent. In this study; in embryos with development arrest, we aimed To investigate the number abnormalities in 13(rd), 16(th), 18(th), 21(st) and 22(nd) chromosomes, To show the correlation between the chromosomal abnormality rates in embryos with developmental arrest and rate of live embryos in same cycle. To investigate the correlation between the apoptosis and the chromosomal number abnormalities in embryos with developmental arrest and sperm cells that are obtained in same cycle. Material and Methods: In twenty embryos with developmental arrest, the number abnormalities of 13(rd), 16(th), 18(th), 21(st) and 22(nd) chromosomes were analyzed with FISH method. The chromosomal numbers of X, Y and 18(th) chromosomes were analyzed with same methods in sperm cells that are obtained from the fathers of embryos at the same cycle. Apoptosis status in sperm cells was assessed with TUNNEL test. Results: The chromosomal number abnormality ratewas 80% in embryos with developmental arrest. No significant correlation was detected between the abnormality rate in embryos and living embryo rate as well as total abnormality rate in sperm cells. No correlation was detected between sperm apoptosis and chromosomal abnormality rates in embryos. However a significant correlation was detected between chromosomal abnormality rate and spermiogram results. Conclusion: In embryos with developmental arrest, chromosomal abnormality rates were higher in 13(rd), 16(th), 18(th), 21(st) and 22(nd) chromosomes. Chromosomal abnormality and apoptosis frequency in sperms were not significantly correlated with chromosomal abnormalities in embryos however a significant correlation was found between spermiogram results of fathers and chromosomal abnormalities in embryos with developmental arrest.
Açıklama
Anahtar Kelimeler
Preimplantation diagnosis, in situ hybridization, fluorescence, blastomeres, aneuploidy, in situ nick-end labeling
Kaynak
Turkiye Klinikleri Tip Bilimleri Dergisi
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
31
Sayı
4
