Bio-active nanoemulsions enriched with gold nanoparticle, marigold extracts and lipoic acid: In vitro investigations

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dc.contributor.authorGuler, Emine
dc.contributor.authorBarlas, F. Baris
dc.contributor.authorYavuz, Murat
dc.contributor.authorDemir, Bilal
dc.contributor.authorGumus, Z. Pinar
dc.contributor.authorBaspinar, Yucel
dc.contributor.authorCoskunol, Hakan
dc.contributor.authorTimur, Suna
dc.date.accessioned2019-10-27T22:13:29Z
dc.date.available2019-10-27T22:13:29Z
dc.date.issued2014
dc.departmentEge Üniversitesien_US
dc.description.abstractA novel and efficient approach for the preparation of enriched herbal formulations was described and their potential applications including wound healing and antioxidant activity (cell based and cell free) were investigated via in vitro cell culture studies. Nigella sativa oil was enriched with Calendula officinalis extract and lipoic acid capped gold nanoparticles (AuNP-LA) using nanoemulsion systems. The combination of these bio-active compounds was used to design oil in water (O/W) and water in oil (W/O) emulsions. The resulted emulsions were characterized by particle size measurements. The phenolic content of each nanoemulsion was examined by using both colorimetric assay and chromatographic analyses. Two different methods containing cell free chemical assay (1-diphenyl-2-picrylhydrazyl method) and cell based antioxidant activity test were used to evaluate the antioxidant capacities. In order to investigate the bio-activities of the herbal formulations, in vitro cell culture experiments, including cytotoxicity, scratch assay, antioxidant activity and cell proliferation were carried out using Vero cell line as a model cell line. Furthermore, to monitor localization of the nanoemulsions after application of the cell culture, the cell images were monitored via fluorescence microscope after FITC labeling. All data confirmed that the enriched N. sativa formulations exhibited better antioxidant and wound healing activity than N. sativa emulsion without any enrichment. In conclusion, the incorporation of AuNP-LA and C officinalis extract into the N. sativa emulsions significantly increased the bio-activities. The present work may support further studies about using the other bio-active agents for the enrichment of herbal preparations to strengthen their activities. (C) 2014 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipState Planning Organization (DPT) of Turkey [0395.STZ.2013-2]; Ege UniversityEge University [2013/FEN/023]; Ege University Aliye Uster FoundationEge Universityen_US
dc.description.sponsorshipThis work was financially supported by the State Planning Organization (DPT) of Turkey through the project no 0395.STZ.2013-2. It was partially supported by Ege University Scientific Research Project (2013/FEN/023) and Ege University Aliye Uster Foundation. SEM (Izmir) was also acknowledged for their support during the HPLC analysis.en_US
dc.identifier.doi10.1016/j.colsurfb.2014.05.026en_US
dc.identifier.endpage306en_US
dc.identifier.issn0927-7765
dc.identifier.issn1873-4367
dc.identifier.pmid25009101en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage299en_US
dc.identifier.urihttps://doi.org/10.1016/j.colsurfb.2014.05.026
dc.identifier.urihttps://hdl.handle.net/11454/49802
dc.identifier.volume121en_US
dc.identifier.wosWOS:000341335800036en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofColloids and Surfaces B-Biointerfacesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectNigella sativa oilen_US
dc.subjectCalendula officinalis extracten_US
dc.subjectGold nanoparticleen_US
dc.subjectLipoic aciden_US
dc.subjectHerbal nanoemulsionsen_US
dc.titleBio-active nanoemulsions enriched with gold nanoparticle, marigold extracts and lipoic acid: In vitro investigationsen_US
dc.typeArticleen_US

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