Effect of microemulsion formulation on biodistribution of Tc-99m-Aprotinin in acute pancreatitis models induced rats

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dc.contributor.authorIlem-Ozdemir, Derya
dc.contributor.authorUstundag-Okur, Neslihan
dc.contributor.authorSenyigit, Zeynep Ay
dc.contributor.authorOruc, Nevin
dc.contributor.authorAsikoglu, Makbule
dc.contributor.authorOzutemiz, Omer
dc.contributor.authorKarasulu, H. Yesim
dc.date.accessioned2019-10-27T11:25:47Z
dc.date.available2019-10-27T11:25:47Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractBackground: Aprotinin is a monomeric globular polypeptide, which derived from bovine lung tissue and theoretically attractive molecule in ameliorating the effects of acute pancreatitis. Acute pancreatitis is an inflammatory condition of the pancreas that is painful and at times deadly. Over the following two decades Aprotinin therapeutic potential on pancreatitis is proven experimentally, its clinical therapeutic success is limited due to low targeting to pancreas. Objective: The aim of this study was to evaluate the biodistribution of Technetium-(99m)(Tc-99m)Aprotinin solution (Tc-99m-Aprotinin-S) and Tc-99m-Aprotinin loaded microemulsion, which was prepared for the aim of treatment for acute pancreatitis. Method: Aprotinin was radiolabeled with Tc-99m. Radiochemical purity was determined with radioactive thin layer chromatography studies. Tc-99m-Aprotinin-S and Tc-99m-Aprotinin loaded microemulsion (Tc-99m-Aprotinin-M) was administered to acute edematous, severe necrotizing pancreatitis and air pouch model induced rats. Tissue distribution of Aprotinin was investigated with gamma scintigraphy and biodistribution studies. Results: Aprotinin was radiolabeled by Tc-99m with high radiochemical purity (95.430 +/- 0.946%). The complex was found to be stable at room temperature up to 6 h. Animal studies have shown that similar to that of other small proteins Aprotinin is accumulated primarily in the kidney. The scintigraphy and biodistribution studies showed that, while i. v. administration of (99m)TcAprotinin-S distributed mostly in kidneys and bladder, Tc-99m-Aprotinin-M, with droplet size of 64.550 +/- 3.217 nm, has high uptake in liver, spleen and pancreas. Conclusion: This might be concluding that microemulsions may be suggested as promising formulations for selectively targeting Aprotinin to pancreas inflammation.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (Tubitak)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [108S083]; T.R. Prime Ministry State Planning Organization FoundationTurkiye Cumhuriyeti Kalkinma Bakanligi [09DPT001]en_US
dc.description.sponsorshipThe authors declare no conflict of interest. The authors alone are responsible for the content and writing of the article. This study was supported by The Scientific and Technological Research Council of Turkey (Tubitak 108S083). The authors also thank to the T.R. Prime Ministry State Planning Organization Foundation (Project Number: 09DPT001).en_US
dc.identifier.doi10.3109/10717544.2016.1145306en_US
dc.identifier.endpage3062en_US
dc.identifier.issn1071-7544
dc.identifier.issn1521-0464
dc.identifier.issue8en_US
dc.identifier.pmid26923781en_US
dc.identifier.startpage3055en_US
dc.identifier.urihttps://doi.org/10.3109/10717544.2016.1145306
dc.identifier.urihttps://hdl.handle.net/11454/33429
dc.identifier.volume23en_US
dc.identifier.wosWOS:000388750400045en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofDrug Deliveryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAprotininen_US
dc.subjectbiodistributionen_US
dc.subjectmicroemulsionen_US
dc.subjectpancreatitisen_US
dc.subjectradiolabeled Aprotininen_US
dc.titleEffect of microemulsion formulation on biodistribution of Tc-99m-Aprotinin in acute pancreatitis models induced ratsen_US
dc.typeArticleen_US

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