Taurine inhibits increased MMP-2 expression in a model of oxidative stress induced by glutathione depletion in rabbit heart

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dc.contributor.authorSevin, Gulnur
dc.contributor.authorOzsarlak-Sozer, Gonen
dc.contributor.authorKeles, Didem
dc.contributor.authorGokce, Goksel
dc.contributor.authorReel, Buket
dc.contributor.authorOzgur, Halil Hakan
dc.contributor.authorOktay, Gulgun
dc.contributor.authorKerry, Zeliha
dc.date.accessioned2019-10-27T21:52:23Z
dc.date.available2019-10-27T21:52:23Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractMatrix metalloproteinase enzymes (MMPs) activated by oxidative stress are involved in the pathogenesis of cardiovascular diseases. Glutathione (GSH) plays an important protective role against oxidatively induced damage in mammalian tissues. We investigated the possible role of gelatinases and the effect of the semiessential amino acid 2-aminoethanesulfonic acid (taurine) in oxidatively induced damage by GSH depletion in rabbit cardiac tissues. Rabbits were treated with buthionine sulfoximine (BSO), an effective GSH-depleting compound. BSO treatment significantly reduced GSH and increased MDA (malondialdehyde) levels. BSO treatment caused significant increase in proMMP-2 levels. MMP-9 (pro and active) expressions were not found in either treated- or untreated heart tissues. TIMP-1(endogenous inhibitor of MMP-9) and MT-MMP1 (endogenous activator of MMP-2) were not affected by BSO. Immunoscoring showed that MMP-2 expression significantly increased in hearts from BSO treated group but MMP-9 antibody did not show any significant positive immunostaining from all groups. Type I procollagen and total collagen did not significantly alter in heart tissues from all treatment groups. Taurine restored the increased MDA and the diminished GSH levels by BSO treatment. Pro MMP-2 expression was prevented by taurine. These results suggest that MMP-2 is a major gelanitase in rabbit hearts under oxidative stress and pharmacological inhibition of MMP-2 activation by taurine could represent a useful strategy for the prevention and/or treatment of different cardiovascular disorders. (C) 2013 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipEge University Scientific Research FundEge University [07ECZ020]en_US
dc.description.sponsorshipWe thank Prof. Dr. Ahmet Ali Kupelioglu for help with the morphological examinations. We are thankful to Dr. Gulperi Oktem for kindly providing us type I procollagen antibody. This work was substantially supported by a grant from the Ege University Scientific Research Fund (07ECZ020, ZK).en_US
dc.identifier.doi10.1016/j.ejphar.2013.02.052en_US
dc.identifier.endpage106en_US
dc.identifier.issn0014-2999
dc.identifier.issue01.Maren_US
dc.identifier.pmid23500209en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage98en_US
dc.identifier.urihttps://doi.org/10.1016/j.ejphar.2013.02.052
dc.identifier.urihttps://hdl.handle.net/11454/47610
dc.identifier.volume706en_US
dc.identifier.wosWOS:000317747700015en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofEuropean Journal of Pharmacologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGelatinasesen_US
dc.subjectOxidative stressen_US
dc.subjectTaurineen_US
dc.subjectBSOen_US
dc.subjectRabbit hearten_US
dc.titleTaurine inhibits increased MMP-2 expression in a model of oxidative stress induced by glutathione depletion in rabbit hearten_US
dc.typeArticleen_US

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