Altered Stem Cell Receptor Activity in the Ovarian Surface Epithelium by Exogenous Zinc and/or Progesterone

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dc.contributor.authorOktem, G.
dc.contributor.authorSahin, C.
dc.contributor.authorDilsiz, O. Y.
dc.contributor.authorDemiray, S. B.
dc.contributor.authorGoker, E. N. T.
dc.contributor.authorTavmergen, E.
dc.date.accessioned2019-10-27T22:27:01Z
dc.date.available2019-10-27T22:27:01Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractBackground: Ovarian surface epithelium (OSE) has the characteristics of a stem cell and the potential for differentiation. Previous studies on this subject have succeeded in deriving oocytes from OSE stem cells, leading to the belief that OSE could be used for infertility treatment. Methods: Each rat (n=10) was subjected to zinc and/or progesterone injection for 5 days after conception. After a 6-day implantation period, ovarian tissues were removed and comprehensive immunohistochemical analysis of stem cell markers was conducted: Sox2, Klf4, Oct3/4, c-Myc, CD117, CD90, SSEA-1 and Notch pathway analysis; Notch1, Jagged1, and Delta1 in the OSE and ovarian stromal cells were evaluated after treatment with zinc, progesterone, or both. Results: Progesterone moderately affected Sox2 expression (p<0.001), while zinc application strongly affected Klf4 and Oct3/4 and immunoreactivity (p<0.001). CD90 immunoreactivity was decreased in the OSE and stroma of the progesterone group (p=0.006) compared with the zinc (p=0.244) and zinc/progesterone groups (p=0.910). On the other hand, SSEA-1 showed moderate staining in the OSE and weak staining in stromal cells in animals treated with zinc (p=0.727), progesterone (p=0.626), and zinc/progesterone (p=0.371), with no differences compared with control. Zinc application affected Notch pathway immunoreactivity, with a significant increase in Notch1 (p=0.0015) and Jagged1 (p<0.001). Conclusions: The expression of putative stem cell markers in the OSE was verified and stem cell receptor activity was raised in the OSE and ovarian stromal cells by zinc and progesterone. Thus, this increased expression allows the therapeutic use of zinc and progesterone in ovary-related infertility and brings a different perspective to reproductive medicine.en_US
dc.identifier.doi10.1055/s-0034-1376975en_US
dc.identifier.endpage258en_US
dc.identifier.issn2194-9379
dc.identifier.issn2194-9387
dc.identifier.issue5en_US
dc.identifier.pmid24992499en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage252en_US
dc.identifier.urihttps://doi.org/10.1055/s-0034-1376975
dc.identifier.urihttps://hdl.handle.net/11454/50541
dc.identifier.volume65en_US
dc.identifier.wosWOS:000354277000005en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherGeorg Thieme Verlag Kgen_US
dc.relation.ispartofDrug Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectstem cellen_US
dc.subjectovaryen_US
dc.subjectzincen_US
dc.subjectprogesteroneen_US
dc.subjectinfertilityen_US
dc.titleAltered Stem Cell Receptor Activity in the Ovarian Surface Epithelium by Exogenous Zinc and/or Progesteroneen_US
dc.typeArticleen_US

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