Toward consensus in the analysis of urinary 8-oxo-7,8-dihydro-2'- deoxyguanosine as a noninvasive biomarker of oxidative stress

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dc.contributor.authorEvans M.D.
dc.contributor.authorOlinski R.
dc.contributor.authorLoft S.
dc.contributor.authorCooke M.S.
dc.contributor.authorRossner Jr. P.
dc.contributor.authorSram R.
dc.contributor.authorHenriksen T.
dc.contributor.authorPoulsen H.E.
dc.contributor.authorWeimann A.
dc.contributor.authorBarbieri A.
dc.contributor.authorSabatini L.
dc.contributor.authorViolante F.
dc.contributor.authorKino S.
dc.contributor.authorOchi T.
dc.contributor.authorSakai K.
dc.contributor.authorTakeuchi M.
dc.contributor.authorKasai H.
dc.contributor.authorMeerman J.H.N.
dc.contributor.authorGackowski D.
dc.contributor.authorRozalski R.
dc.contributor.authorSiomek A.
dc.contributor.authorHalliwell B.
dc.contributor.authorJenner A.M.
dc.contributor.authorWang H.
dc.contributor.authorCerda C.
dc.contributor.authorSaez G.
dc.contributor.authorHaghdoost S.
dc.contributor.authorSvoboda P.
dc.contributor.authorHu C.-W.
dc.contributor.authorChao M.-R.
dc.contributor.authorPeng K.-Y.
dc.contributor.authorShih W.-C.
dc.contributor.authorWu K.-Y.
dc.contributor.authorOrhan H.
dc.contributor.authorIstanbullu N.S.
dc.contributor.authorMistry V.
dc.contributor.authorFarmer P.B.
dc.contributor.authorSandhu J.
dc.contributor.authorSingh R.
dc.contributor.authorCortez C.
dc.contributor.authorSu Y.
dc.contributor.authorSantella R.M.
dc.contributor.authorLambert P.
dc.contributor.authorSmith R.
dc.date.accessioned2019-10-26T22:38:55Z
dc.date.available2019-10-26T22:38:55Z
dc.date.issued2010
dc.departmentEge Üniversitesien_US
dc.description.abstractOf the DNA-derived biomarkers of oxidative stress, urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is the most frequently measured. However, there is significant discrepancy between chromatographic and immunoassay approaches, and intratechnique agreement among all available chromatography-based assays and ELISAs is yet to be established. This is a significant obstacle to their use in large molecular epidemiological studies. To evaluate the accuracy of intra/intertechnique and interlaboratory measurements, samples of phosphate buffered saline and urine, spiked with different concentrations of 8-oxoG, together with a series of urine samples from healthy individuals were distributed to ESCULA members. All laboratories received identical samples, including 2 negative controls that contained no added 8-oxodG. Data were returned from 17 laboratories, representing 20 methods, broadly classified as mass spectrometric (MS), electrochemical detection (EC), or enzyme-linked immunosorbant assay (ELISA). Overall, there was good within-technique agreement, with the majority of laboratories' results lying within 1 SD of their consensus mean. However, ELISA showed more within-technique variation than did the chromatographic techniques and, for the urine samples, reported higher values. Bland-Altman plots revealed good agreement between MS and EC methods but concentration-dependent deviation for ELISA. All methods ranked urine samples according to concentration similarly. Creatinine levels are routinely used as a correction factor for urine concentration, and therefore we also conducted an interlaboratory comparison of methods for urinary creatinine determination, in which the vast majority of values lay within 1 SD of the consensus value, irrespective of the analysis procedure. This study reveals greater consensus than previously expected, although concern remains over ELISA. © FASEB.en_US
dc.identifier.doi10.1096/fj.09-147124en_US
dc.identifier.endpage1260en_US
dc.identifier.issn0892-6638
dc.identifier.issue4en_US
dc.identifier.pmid19966135en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1249en_US
dc.identifier.urihttps://doi.org/10.1096/fj.09-147124
dc.identifier.urihttps://hdl.handle.net/11454/19952
dc.identifier.volume24en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofFASEB Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDiseaseen_US
dc.subjectDNA damageen_US
dc.subjectDNA repairen_US
dc.subjectElectrochemical detectionen_US
dc.subjectImmunoassayen_US
dc.subjectMass spectrometryen_US
dc.titleToward consensus in the analysis of urinary 8-oxo-7,8-dihydro-2'- deoxyguanosine as a noninvasive biomarker of oxidative stressen_US
dc.typeArticleen_US

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