Identification of benzoin obtained from calli of Styrax officinalis by HPLC

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dc.contributor.authorDemiray, Hatice
dc.contributor.authorEsiz Dereboylu, Aylin
dc.contributor.authorYazici, Zekiye Isin
dc.contributor.authorKarabey, Fatih
dc.date.accessioned2019-10-27T21:54:04Z
dc.date.available2019-10-27T21:54:04Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractThe stem tissue of Styrax officinalis L. distributed in West Anatolia was induced with agents such as boric acid and cocarboxylase (thiamine diphosphate), extensive stimulators of resin channels, in order to increase the amount of benzoin volatile oil. While the benzoin content was 120% in the induction medium to which excess boron and niacin were added, it increased to 231% when cocarboxylase (thiamine diphosphate) was added to the medium. Benzoin content of Styrax from petiole calli was 166%. HPLC-DAD results revealed that benzoin resin was present in 90% of the stems of Styrax officinalis distributed in West Anatolia. The major components of excess boron-and niacin-induced stem calli were hexane (58.33%), 3-methyl 2-pentene (16.10%), and cyclohexane (8.88%). Hexane (62%), methyl cyclopentane (19.09%), cyclohexane (12.04%), 2-hexanone (0.04%), ethylbenzene (0.03%), and benzene and 1-chloro-2-methylpropyl benzene (propene) were identified by the cocarboxylase application and GC-MS method. With enzyme application, while the percentages of decane and benzyl alcohol decreased, the cyclohexane ratio increased to 12.04%. Acetone (0.03%), ethyl acetate (4.10%), and dichloro methane (0.17%) contents were high as well.en_US
dc.identifier.doi10.3906/bot-1209-16
dc.identifier.endpage963en_US
dc.identifier.issn1300-008X
dc.identifier.issue5en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage956en_US
dc.identifier.urihttps://doi.org/10.3906/bot-1209-16
dc.identifier.urihttps://hdl.handle.net/11454/48016
dc.identifier.volume37en_US
dc.identifier.wosWOS:000324167200017en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technical Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Botanyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectStyrax officinalisen_US
dc.subjectstem callien_US
dc.subjectbenzoinen_US
dc.subjectcocarboxylaseen_US
dc.subjectboronen_US
dc.titleIdentification of benzoin obtained from calli of Styrax officinalis by HPLCen_US
dc.typeArticleen_US

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