Comparison of the "ProDect BCS RV CHIP" assay with the combination of shell vial cell culture and immunofluorescence antibody test for the detection of respiratory viruses

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dc.contributor.authorCicek, Candan
dc.contributor.authorGulen, Figen
dc.contributor.authorKaratas, Eylem
dc.contributor.authorDemir, Esen
dc.contributor.authorTanac, Remziye
dc.contributor.authorBacakoglu, Feza
dc.contributor.authorBilgic, Altinay
dc.date.accessioned2019-10-27T19:33:13Z
dc.date.available2019-10-27T19:33:13Z
dc.date.issued2007
dc.departmentEge Üniversitesien_US
dc.description.abstractIn the present study, a multiplex reverse transcriptase polymerase chain reaction combined with a chip hybridization assay (ProDect BCS RV CHIP) was evaluated as an alternative to the combination of immunofluorescent antibody test and shell vial cell culture considered as gold standard for the detection of respiratory viruses. Among 100 specimens, 40 were positive using the combination of immunofluorescent antibody test and shell vial cell culture assay in which 9 of them were infected by two different viruses (27 parainfluenza virus type 3, 10 adenovirus, 9 respiratory, syncytial virus. 2 influenza type B, and 1 influenza type A). ProDect BCS RV CHIP detected only 10 positive specimens in which one of them was infected by two different viruses (5 respiratory syncytial virus, 3 parainfluenza virus type 3, 2 adenovirus, and I influenza virus type B). The sensitivity. specificity, PPV. NPV and diagnostic accuracy of ProDect BCS RV CHIP were 25.0%, 100%, 100%, 66.6%, and 70.0%, respectively, compared to the combination of shell vial cell culture and immunofluorescent antibody test. As a result, the specificity of ProDect BCS RV CHIP is high. however. the sensitivity (25%) of the assay is not sufficient for routine laboratory use. (C) 2007 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.jviromet.2007.03.003en_US
dc.identifier.endpage168en_US
dc.identifier.issn0166-0934
dc.identifier.issue2en_US
dc.identifier.pmid17433455en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage161en_US
dc.identifier.urihttps://doi.org/10.1016/j.jviromet.2007.03.003
dc.identifier.urihttps://hdl.handle.net/11454/39434
dc.identifier.volume143en_US
dc.identifier.wosWOS:000248110000006en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofJournal of Virological Methodsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectrespiratory virusesen_US
dc.subjectshell vial cell cultureen_US
dc.subjectimmunofluorescent antibody testen_US
dc.subjectmultiplex RT-PCRen_US
dc.titleComparison of the "ProDect BCS RV CHIP" assay with the combination of shell vial cell culture and immunofluorescence antibody test for the detection of respiratory virusesen_US
dc.typeArticleen_US

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