Concanavalin A immobilized poly(ethylene glycol dimethacrylate) based affinity cryogel matrix and usability of invertase immobilization

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dc.contributor.authorUygun, Murat
dc.contributor.authorUygun, Deniz Aktas
dc.contributor.authorOzcaliskan, Emir
dc.contributor.authorAkgol, Sinan
dc.contributor.authorDenizli, Adil
dc.date.accessioned2019-10-27T21:44:05Z
dc.date.available2019-10-27T21:44:05Z
dc.date.issued2012
dc.departmentEge Üniversitesien_US
dc.description.abstractConcanavalin A (Con A) immobilized supermacroporous poly(ethylene glycol dimethacrylate) [poly(EGDMA)] monolithic cryogel column was prepared by radical cryocopolymerization of EGDMA as a monomer and N,N'-methylene-bisacrylamide as a crosslinker. Bioligand Con A was then immobilized by covalent binding onto poly(EGDMA) cryogel via glutaraldehyde activation [Con A-poly(EGDMA)]. Con A-poly(EGDMA) cryogel was characterized by swelling studies and scanning electron microscopy. The monolithic cryogel contained a continuous polymeric matrix having interconnected pores of 10-50 mu m size. The equilibrium swelling degree of the cryogel was 15.01 g H2O/g dry cryogel. Con A-poly(EGDMA) cryogel was used in the adsorption/desorption of invertase from aqueous solutions. The maximum amount of invertase adsorption from aqueous solution in acetate buffer was 55.45 mg/g polymer at pH 5.0. Con A-poly(EGDMA) cryogels were used for repetitive adsorption/desorption of invertase without noticeable loss in invertase adsorption capacity after 10 cycles. (c) 2012 Elsevier By. All rights reserved.en_US
dc.identifier.doi10.1016/j.jchromb.2012.01.014en_US
dc.identifier.endpage78en_US
dc.identifier.issn1570-0232
dc.identifier.pmid22309777en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage73en_US
dc.identifier.urihttps://doi.org/10.1016/j.jchromb.2012.01.014
dc.identifier.urihttps://hdl.handle.net/11454/47193
dc.identifier.volume887en_US
dc.identifier.wosWOS:000302049600011en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofJournal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCryogelen_US
dc.subjectAffinity separationen_US
dc.subjectInvertaseen_US
dc.subjectConcanavalin Aen_US
dc.subjectEGDMAen_US
dc.titleConcanavalin A immobilized poly(ethylene glycol dimethacrylate) based affinity cryogel matrix and usability of invertase immobilizationen_US
dc.typeArticleen_US

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