DETERMINATION OF FETAL RHESUS D STATUS BY MATERNAL PLASMA DNA ANALYSIS

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dc.contributor.authorAykut, A.
dc.contributor.authorOnay, H.
dc.contributor.authorSagol, S.
dc.contributor.authorGunduz, C.
dc.contributor.authorÖzkınay, Ferda
dc.contributor.authorCogulu, O.
dc.date.accessioned2019-10-27T21:53:37Z
dc.date.available2019-10-27T21:53:37Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractIn this study, we assessed the feasibility of fetal RhD genotyping by analysis of cell-free fetal DNA(cffDNA) extracted from plasma samples of Rhesus (Rh) D-negative pregnant women by using real-time polymerase chain reaction (PCR). Fetal genotyping was performed on 30 RhD-negative women between 9 and 39 weeks of gestation who were referred to us for invasive testing [amniocentesis/chorionic villi sampling (CVS)]. The fetal RHD genotype was determined based on real-time PCR method. Exons 7 and 10 of the RHD and SRY genes were targeted. Among the pregnant women, 12 were carrying male and 17 were carrying female fetuses. Out of 29 pregnant women, 21 had RhD-positive and nine had RhD-negative fetuses. One sample) case 12, whose blood group was found to be AB Rh [+] (was excluded due to controversial results from repeated serological analyses. All prenatal results were in concordance with postnatal RhD status and fetal sex without false-positive or -negative results. Performing real-time PCR on cffDNA showed accurate, efficient and reliable results, allowing rapid and high throughput non invasive determination of fetal sex and RhD status in clinical samples.en_US
dc.identifier.doi10.2478/bjmg-2013-0029en_US
dc.identifier.endpage38en_US
dc.identifier.issn1311-0160
dc.identifier.issue2en_US
dc.identifier.pmid24778561en_US
dc.identifier.startpage33en_US
dc.identifier.urihttps://doi.org/10.2478/bjmg-2013-0029
dc.identifier.urihttps://hdl.handle.net/11454/47930
dc.identifier.volume16en_US
dc.identifier.wosWOS:000332942700005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMacedonian Acad Sciences Artsen_US
dc.relation.ispartofBalkan Journal of Medical Geneticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectRhesus D (RhD)en_US
dc.subjectNon invasive prenatal diagnosisen_US
dc.titleDETERMINATION OF FETAL RHESUS D STATUS BY MATERNAL PLASMA DNA ANALYSISen_US
dc.typeArticleen_US

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