Extended Snake Venomics by Top-Down In-Source Decay: Investigating the Newly Discovered Anatolian Meadow Viper Subspecies, Vipera anatolica senliki

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dc.contributor.authorHempel, Benjamin-Florian
dc.contributor.authorDamm, Maik
dc.contributor.authorGocmen, Bayram
dc.contributor.authorKaris, Mert
dc.contributor.authorNalbantsoy, Ayse
dc.contributor.authorKini, R. Manjunatha
dc.contributor.authorSuessmuth, Roderich D.
dc.date.accessioned2020-12-01T12:01:40Z
dc.date.available2020-12-01T12:01:40Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractHerein, we report on the venom proteome of Vipera anatolica senliki, a recently discovered and hitherto unexplored subspecies of the critically endangered Anatolian meadow viper endemic to the Antalya Province of Turkey. Integrative venomics, including venom gland transcriptomics as well as complementary bottom-up and top-down proteomics analyses, were applied to fully characterize the venom of V. a. senliki. Furthermore, the classical top-down venomics approach was extended to elucidate the venom proteome by an alternative in-source decay (ISD) proteomics workflow using the reducing matrix 1,5-diaminonaphthalene. Top-down ISD proteomics allows for disulfide bond counting and effective de novo sequencing-based identification of highmolecular-weight venom constituents, both of which are difficult to achieve by commonly established top-down approaches. Venom gland transcriptome analysis identified 96 toxin transcript annotations from 18 toxin families. Relative quantitative snake venomics revealed snake venom metalloproteinases (42.9%) as the most abundant protein family, followed by several less dominant toxin families. Online mass profiling and top-down venomics provide a detailed insight into the venom proteome of V. a. senliki and facilitate a comparative analysis of venom variability for the closely related subspecies, Vipera anatolica anatolica.en_US
dc.description.sponsorshipSouth East Asian Biodiversity Genomics (SEABIG) [R-154-000-648646]en_US
dc.description.sponsorshipWe dedicate this paper to the memory of Professor Bayram Gocmen, who lost his fight against cancer. He was an outstanding teacher, a good friend and colleague, and loved by his family. Further, we would like to thank Christoph Weise from the Freie Universitat Berlin for the permission and access to the MALDI device as well as for showing us the basic principles for ISD-MALDI. We also want to thank Joshua Baal for providing us with pictures of V. b. berus and Bayram Gocmen for all remaining pictures in this study. We thank Murat Senlik (subspecies name is derived from his surname) and M. Anil Og.uz for their assistance during the fieldwork. the transcriptome analysis was funded by a South East Asian Biodiversity Genomics (SEABIG) grant no. R-154-000-648646.en_US
dc.identifier.doi10.1021/acs.jproteome.9b00869en_US
dc.identifier.endpage1749en_US
dc.identifier.issn1535-3893
dc.identifier.issn1535-3907
dc.identifier.issue4en_US
dc.identifier.pmid32073270en_US
dc.identifier.scopus2-s2.0-85082979450en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1731en_US
dc.identifier.urihttps://doi.org/10.1021/acs.jproteome.9b00869
dc.identifier.urihttps://hdl.handle.net/11454/62486
dc.identifier.volume19en_US
dc.identifier.wosWOS:000526337800032en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmer Chemical Socen_US
dc.relation.ispartofJournal of Proteome Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAnatolian meadow viperen_US
dc.subjectbottom-upen_US
dc.subjectin-source decayen_US
dc.subjectintact mass profilingen_US
dc.subjectvenomicsen_US
dc.subjecttranscriptomicsen_US
dc.subjectproteomicsen_US
dc.subjecttop-downen_US
dc.subjectVipera anatolica senlikien_US
dc.subjectViperidaeen_US
dc.titleExtended Snake Venomics by Top-Down In-Source Decay: Investigating the Newly Discovered Anatolian Meadow Viper Subspecies, Vipera anatolica senlikien_US
dc.typeArticleen_US

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