Cytotoxicity and genotoxicity evaluations of oleic acid and conjugated linoleic acid

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dc.contributor.authorKizilsahin, Sadi
dc.contributor.authorKarayildirim, Cinel Koksal
dc.contributor.authorBakan, Buket
dc.contributor.authorNalbantsoy, Ayse
dc.contributor.authorYavasoglu, Nefise Karabay
dc.date.accessioned2023-01-12T20:12:33Z
dc.date.available2023-01-12T20:12:33Z
dc.date.issued2022
dc.departmentN/A/Departmenten_US
dc.description.abstractBackground and Aims: Oleic acid (OLA) and conjugated linoleic acid (CLA) occur in dairy products and meats and are also widespread at lower levels in many other foodstuffs. It is known that OLA and CLA are very bioactive compounds with substantially anti-carcinogenic effects. The objective of this study was to evaluate the cytotoxic potentials of OLA and CLA which were tested against cancerous and non-cancerous cell lines and to determine their genotoxicity. Methods: The cytotoxic activities of OLA and CLA against to cancer cell lines (U-87-MG, A549, MCF-7, CaCo-2, HeLa and PC-3) and a control cell line (HEK293) were assessed by MIT assay. Ames MPFtm mutagenicity assay on 4 strains (TA98, TA100, TA 1535 and TA 1537) of Salmonella typhimurium was used for genotoxicity determination. Results: CLA showed cytotoxic activity on PC-3 cells, while OLA was created on A549 and PC-3 cell lines with the IC50 of 20 nM and 15 nM, respectively. No cytotoxic activity was observed on MCF-7, HeLa, U-87-MG, and CaCo-2 cells with the administered doses of OLA and CLA. It has been proved that OLA and CLA are characterized by a high cytotoxic activity towards cancer cells, as observed in the cell line test. There was no evidence for a mutagenic effect of OLA and CLA in the Ames test, with or without metabolic activation (S9) against Salmonella typhimurium strains. Conclusion: These in vitro test results indicate that these fatty acids can be considered a beneficial dietary supplement for enhancing anti-cancer therapy.en_US
dc.description.sponsorshipEge University Research Fund Project [2013/FEN/051, 13/ILAM/001]en_US
dc.description.sponsorshipThe study was supported by Ege University Research Fund Project (2013/FEN/051 and 13/ILAM/001).en_US
dc.identifier.doi10.26650/IstanbulJPharm.2022.1023891
dc.identifier.endpage19en_US
dc.identifier.issn2587-2087
dc.identifier.issue1en_US
dc.identifier.startpage14en_US
dc.identifier.trdizinid1126908en_US
dc.identifier.urihttps://doi.org/10.26650/IstanbulJPharm.2022.1023891
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1126908
dc.identifier.urihttps://hdl.handle.net/11454/78323
dc.identifier.volume52en_US
dc.identifier.wosWOS:000793925000003en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherIstanbul Univ, Fac Pharmacyen_US
dc.relation.ispartofIstanbul Journal Of Pharmacyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectFatty aciden_US
dc.subjectConjugated linoleic aciden_US
dc.subjectOleic aciden_US
dc.subjectCytotoxicityen_US
dc.subjectMutagenicityen_US
dc.subjectBreast-Canceren_US
dc.subjectGrowthen_US
dc.titleCytotoxicity and genotoxicity evaluations of oleic acid and conjugated linoleic aciden_US
dc.typeArticleen_US

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