Development of transition metal oxide platforms for aptasensing of PSA in cell cultures

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dc.authorid0000-0001-8506-1491
dc.authorid0000-0001-5029-9256
dc.authorid0000-0002-6189-1818
dc.authorid0000-0002-1940-7279
dc.contributor.authorKirlangic, Irem Aydin
dc.contributor.authorUner, Goklem
dc.contributor.authorKara, Pinar
dc.contributor.authorKirmizibayrak, Petek Ballar
dc.contributor.authorErtas, Fatma Nil
dc.date.accessioned2025-05-05T07:28:34Z
dc.date.available2025-05-05T07:28:34Z
dc.date.issued2024
dc.departmentEge Üniversitesi, Eczacılık Fakültesi
dc.description.abstractIn this study, a novel aptasensor based on a transition metal oxide-modified pencil graphite electrode (PGE) was developed for the diagnosis of early-stage prostate cancer (PCa) via monitoring the prostate-specific antigen (PSA), which is the main biomarker for PCa. Single-use PGEs modified with pulsed deposited manganese oxide (MnOx) film were used to attach the amino-terminated aptamer specific to the PSA via carbodiimide chemistry. The designed aptasensor was placed in an electrochemical cell containing ferri/ferrocyanide ions as a redox probe to measure the charge transfer resistances (Rct) of the electrode surface by electrochemical impedance spectroscopy (EIS) to follow the response of each modification step. The effect of the medium pH on the ionic structure of the aptamer molecule according to its pI value and, thus, the reversing of the direction of the response (Delta Rct) by the pH change was also discussed. The level of PSA secreted from PCa cells was investigated using impedimetric transduction. The specificity of the aptasensor was validated through selectivity studies against non-specific tumor markers like VEGF and different cancer cell lines including breast cancer and androgen-insensitive prostate cancer. The developed system showcases a label-free, fast, specific, and cost-effective approach for PSA detection, highlighting the importance of medium pH and the electrostatic environment on the aptamer's response. Our work emphasizes the potential for such aptasensors in clinical diagnostics and paves the way for further exploration into using transition metal oxides in biosensing applications.
dc.identifier.citationKirlangiç, I. A., Üner, G., Kara, P., Kirmizibayrak, P. B., & Ertaş, F. N. (2024). Development of transition metal oxide platforms for aptasensing of PSA in cell cultures. Analytical and Bioanalytical Chemistry, 416(28), 6421-6435.
dc.identifier.doi10.1007/s00216-024-05529-w
dc.identifier.endpage6435
dc.identifier.issn16182642
dc.identifier.issue28
dc.identifier.pmid39352470
dc.identifier.scopusqualityQ1
dc.identifier.startpage6421
dc.identifier.urihttps://doi.org/10.1007/s00216-024-05529-w
dc.identifier.urihttps://hdl.handle.net/11454/117188
dc.identifier.volume416
dc.identifier.wosWOS:001326688800002
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKirlangic, Irem Aydin
dc.institutionauthorKara, Pinar
dc.institutionauthorKirmizibayrak, Petek Ballar
dc.institutionauthorErtas, Fatma Nil
dc.institutionauthorid0000-0001-8506-1491
dc.institutionauthorid0000-0001-5029-9256
dc.institutionauthorid0000-0002-6189-1818
dc.institutionauthorid0000-0002-1940-7279
dc.language.isoen
dc.publisherSpringer Heidelberg
dc.relation.ispartofAnalytical and Bioanalytical Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAptasensor
dc.subjectEIS
dc.subjectManganese oxide
dc.subjectPSA
dc.titleDevelopment of transition metal oxide platforms for aptasensing of PSA in cell cultures
dc.typeArticle

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