Dye-Loaded Polymersome-Based Lateral Flow Assay: Rational Design of a COVID-19 Testing Platform by Repurposing SARS-CoV-2 Antibody Cocktail and Antigens Obtained from Positive Human Samples

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dc.authoridGhorbanizamani, Faezeh/0000-0001-8541-0540
dc.authoridMOULAHOUM, Hichem/0000-0003-3934-6415
dc.authoridTimur, Suna/0000-0002-1981-7577
dc.authoridDurmus, Ceren/0000-0002-8648-0505
dc.authoridTOK, Kerem/0000-0002-7244-3949
dc.authoridBALABAN HANOGLU, Simge/0000-0002-4540-1882
dc.authorscopusid57199103659
dc.authorscopusid57220178117
dc.authorscopusid57188963997
dc.authorscopusid55935858800
dc.authorscopusid57224479557
dc.authorscopusid57210729367
dc.authorscopusid6602258835
dc.authorwosidGhorbanizamani, Faezeh/ABE-3800-2021
dc.authorwosidÇiçek, Candan/ABH-1161-2021
dc.authorwosidEvran, Serap/AFU-1537-2022
dc.authorwosidMOULAHOUM, Hichem/DIC-3335-2022
dc.authorwosidTimur, Suna/CAG-0914-2022
dc.authorwosidTok, Kerem/AAG-6529-2021
dc.contributor.authorGhorbanizamani, Faezeh
dc.contributor.authorTok, Kerem
dc.contributor.authorMoulahoum, Hichem
dc.contributor.authorHarmanci, Duygu
dc.contributor.authorHanoglu, Simge Balaban
dc.contributor.authorDurmus, Ceren
dc.contributor.authorZihnioglu, Figen
dc.date.accessioned2023-01-12T19:58:37Z
dc.date.available2023-01-12T19:58:37Z
dc.date.issued2021
dc.departmentN/A/Departmenten_US
dc.description.abstractThe global pandemic of COVID-19 continues to be an important threat, especially with the fast transmission rate observed after the discovery of novel mutations. In this perspective, prompt diagnosis requires massive economical and human resources to mitigate the disease. The current study proposes a rational design of a colorimetric lateral flow immunoassay (LFA) based on the repurposing of human samples to produce COVID-19-specific antigens and antibodies in combination with a novel dye-loaded polymersome for naked-eye detection. A group of 121 human samples (61 serums and 60 nasal swabs) were obtained and analyzed by RT-PCR and ELISA. Pooled samples were used to purify antibodies using affinity chromatography, while antigens were purified via magnetic nanoparticles-based affinity. The purified proteins were confirmed for their specificity to COVID-19 via commercial LFA, ELISA, and electrochemical tests in addition to sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. Polymersomes were prepared using methoxy polyethylene glycol-b-polycaprolactone (mPEG-b-PCL) diblock copolymers and loaded with a Coomassie Blue dye. The polymersomes were then functionalized with the purified antibodies and applied for the preparation of two types of LFA (antigen test and antibody test). Overall, the proposed diagnostic tests demonstrated 93 and 92.2% sensitivity for antigen and antibody tests, respectively. The repeatability (92-94%) and reproducibility (96-98%) of the tests highlight the potential of the proposed LFA. The LFA test was also analyzed for stability, and after 4 weeks, 91-97% correct diagnosis was observed. The current LFA platform is a valuable assay that has great economical and analytical potential for widespread applications.en_US
dc.description.sponsorshipEGE University, Research Foundation [TOA2020-21862]; Republic of Turkey, Ministry of Development [2010K120810/2020K12150700, 2016K121190]en_US
dc.description.sponsorshipWe send our acknowledgment to the staff of EGE University Hospital who are on the front line fighting against the COVID19 pandemic. FTIR and SEM analyses were performed at EGE MATAL (Ege University/Izmir). EGE University, Research Foundation was acknowledged for the financial support (TOA2020-21862). Republic of Turkey, Ministry of Development provided the support for the infrastructure of EGE MATAL (Ege University/Izmir) via 2010K120810/2020K12150700 and 2016K121190 grants.en_US
dc.identifier.doi10.1021/acssensors.1c00854
dc.identifier.endpage2997en_US
dc.identifier.issn2379-3694
dc.identifier.issue8en_US
dc.identifier.pmid34270230en_US
dc.identifier.scopus2-s2.0-85111489314en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage2988en_US
dc.identifier.urihttps://doi.org/10.1021/acssensors.1c00854
dc.identifier.urihttps://hdl.handle.net/11454/76971
dc.identifier.volume6en_US
dc.identifier.wosWOS:000692037700023en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmer Chemical Socen_US
dc.relation.ispartofAcs Sensorsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCOVID-19en_US
dc.subjectin vitro diagnosticsen_US
dc.subjectpoint-of-care (POC) platformen_US
dc.subjectlateral flow assayen_US
dc.subjectdye-loaded polymersomeen_US
dc.subjectsample repurposingen_US
dc.subjectViral Loaden_US
dc.subjectIdentificationen_US
dc.subjectAmplificationen_US
dc.titleDye-Loaded Polymersome-Based Lateral Flow Assay: Rational Design of a COVID-19 Testing Platform by Repurposing SARS-CoV-2 Antibody Cocktail and Antigens Obtained from Positive Human Samplesen_US
dc.typeArticleen_US

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