Optimization of beta-Galactosidase Production in Stirred Tank Bioreactor Using Kluyveromyces lactis NRRL Y-8279

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dc.contributor.authorDagbagli, Seval
dc.contributor.authorGoksungur, Yekta
dc.date.accessioned2019-10-27T20:47:44Z
dc.date.available2019-10-27T20:47:44Z
dc.date.issued2009
dc.departmentEge Üniversitesien_US
dc.description.abstractThis paper investigates the production and optimization of beta-galactosidase enzyme using synthetic medium by Kluyveromyces lactis NRRL Y-8279 in stirred tank bioreactor. Response surface methodology was used to investigate the effects of fermentation parameters on P-galactosidase enzyme production. Maximum specific enzyme activity of 4,622.7 U/g was obtained at the optimum levels of process variables (aeration rate 2.21 vvm, agitation speed 173.4 rpm, initial sugar concentration 33.8 g/L, incubation time 24.0 hr). The optimum temperature and pH of the P-galactosidase enzyme produced under optimized conditions were 37 degrees C and pH 7.0, respectively. The enzyme was stable over a pH range of 6.0-7.5 and a temperature range of 25-37 degrees C. The K-m and V-max values for O-nitrophenol-beta-D-galactopyranoside (ONPG) were 1.20 mM and 1,000 mu mol/min.mg protein, respectively. The response surface methodology was found to be useful in optimizing and determining the interactions among process variables in beta-galactosidase enzyme production. Hence, this study fulfills the lack of using mathematical and statistical techniques in optimizing beta-galactosidase enzyme production in stirred tank bioreactor.en_US
dc.description.sponsorshipState Planning Organization (Turkey)Turkiye Cumhuriyeti Kalkinma Bakanligi [DPT.2005 K120570]; Scientific and Technical Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TOVAG 104 O 270]en_US
dc.description.sponsorshipThis research was supported by the State Planning Organization (Turkey) through Project No: DPT.2005 K120570 and Scientific and Technical Research Council of Turkey (TUBITAK) through Project No: TOVAG 104 O 270.en_US
dc.identifier.endpage1350en_US
dc.identifier.issn1226-7708
dc.identifier.issn2092-6456
dc.identifier.issn1226-7708en_US
dc.identifier.issn2092-6456en_US
dc.identifier.issue6en_US
dc.identifier.startpage1342en_US
dc.identifier.urihttps://hdl.handle.net/11454/42606
dc.identifier.volume18en_US
dc.identifier.wosWOS:000273438500006en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherKorean Society Food Science & Technology-Kosfosten_US
dc.relation.ispartofFood Science and Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectstirred tank bioreactoren_US
dc.subjectbeta-galactosidaseen_US
dc.subjectKlulyveromyces lactisen_US
dc.subjectresponse surface methodologyen_US
dc.subjectsynthetic mediumen_US
dc.titleOptimization of beta-Galactosidase Production in Stirred Tank Bioreactor Using Kluyveromyces lactis NRRL Y-8279en_US
dc.typeArticleen_US

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