Development of liquid crystal biosensor for the detection of amyloid beta-42 levels associated with Alzheimer's disease

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dc.authorscopusid55014328200
dc.authorscopusid57223130963
dc.authorscopusid11141742600
dc.contributor.authorKemiklioglu, Emine
dc.contributor.authorTuncgovde, Ebru Busra
dc.contributor.authorOzsarlak-Sozer, Gonen
dc.date.accessioned2023-01-12T19:55:09Z
dc.date.available2023-01-12T19:55:09Z
dc.date.issued2021
dc.departmentN/A/Departmenten_US
dc.description.abstractThis study represents the development of a biosensor which is based on the liquid crystal (LC) orientation as a function of the peptide concentration to detect an amyloid-beta-42 (A beta 42) antibody-antigen binding events. The A beta 42 peptide binds to the A beta 42 antibody forming an immunocomplex which is immobilized on the Dimethyloctadecyl[3-(trimethoxysilyl)propyl] ammonium chloride (DMOAP) coated surface. The disturbed orientation of LCs as a result of the binding of the formed immunocomplex was observed using the polarized optical microscope (POM) as a function of decreasing A beta 42 peptide concentration from 1000 to 1 pg/ml. The concentration, as low as 1 pg/ml of A beta 42 peptide was able to be successfully detected in our system. Apolipoprotein E4 (ApoE4), that specifically bound to the A beta 42 peptide, was added into the system and a remarkable change in reflection spectra of samples was observed with increasing A beta 42 peptide concentration. The concentration of ApoE4 protein was detected in the range of 0.1-30 nM by this system due to the interaction between the two proteins. (C) 2021, The Society for Biotechnology, Japan. All rights reserved.en_US
dc.description.sponsorshipScientific Research Project Office of Manisa Celal Bayar University [2019-055]en_US
dc.description.sponsorshipThis project was financially supported by Scientific Research Project Office of Manisa Celal Bayar University under grant number 2019-055.en_US
dc.identifier.doi10.1016/j.jbiosc.2021.03.016
dc.identifier.endpage94en_US
dc.identifier.issn1389-1723
dc.identifier.issn1347-4421
dc.identifier.issue1en_US
dc.identifier.pmid33934978en_US
dc.identifier.scopus2-s2.0-85104936923en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage88en_US
dc.identifier.urihttps://doi.org/10.1016/j.jbiosc.2021.03.016
dc.identifier.urihttps://hdl.handle.net/11454/76624
dc.identifier.volume132en_US
dc.identifier.wosWOS:000662887900012en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSoc Bioscience Bioengineering Japanen_US
dc.relation.ispartofJournal of Bioscience and Bioengineeringen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLiquid crystalen_US
dc.subjectDMOAP coatingen_US
dc.subjectLiquid crystal biosensoren_US
dc.subjectAmyloid beta-42en_US
dc.subjectApolipoprotein E4en_US
dc.subjectApolipoprotein-Een_US
dc.subjectProteinen_US
dc.subjectPeptidesen_US
dc.subjectAmplificationen_US
dc.subjectAggregationen_US
dc.subjectDiagnosisen_US
dc.subjectBiomarkeren_US
dc.subjectSurfacesen_US
dc.titleDevelopment of liquid crystal biosensor for the detection of amyloid beta-42 levels associated with Alzheimer's diseaseen_US
dc.typeArticleen_US

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