Wound healing effects of collagen-laminin dermal matrix impregnated with resveratrol loaded hyaluronic acid-DPPC microparticles in diabetic rats

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dc.contributor.authorGokce, Evren H.
dc.contributor.authorTanriverdi, Sakine Tuncay
dc.contributor.authorEroglu, Ipek
dc.contributor.authorTsapis, Nicolas
dc.contributor.authorGokce, Goksel
dc.contributor.authorTekmen, Isil
dc.contributor.authorFattal, Elias
dc.contributor.authorOzer, Ozgen
dc.date.accessioned2019-10-27T11:05:12Z
dc.date.available2019-10-27T11:05:12Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractAn alternative formulation for the treatment of diabetic foot wounds that heal slowly is a requirement in pharmaceutical field. The aim of this study was to develop a dermal matrix consisting of skin proteins and lipids with an antioxidant that will enhance healing and balance the oxidative stress in the diabetic wound area due to the high levels of glucose. Thus a novel three dimensional collagen-laminin porous dermal matrix was developed by lyophilization. Resveratrol-loaded hyaluronic acid and dipalmitoylpho sphatidylcholine microparticles were combined with this dermal matrix. Characterization, in vitro release, microbiological and in vivo studies were performed. Spherical microparticles were obtained with a high RSV encapsulation efficacy. The microparticles were well dispersed in the dermal matrix from the surface to deeper layers. Collagenase degraded dermal matrix, however the addition of RSV loaded microparticles delayed the degradation time. The release of RSV was sustained and reached 70% after 6 h. Histological changes and antioxidant parameters in different treatment groups were investigated in full-thickness excision diabetic rat model. Collagen fibers were intense and improved by the presence of formulation without any signs of inflammation. The highest healing score was obtained with the dermal matrix impregnated with RSV-microparticles with an increased antioxidant activity. Collagenlaminin dermal matrix with RSV-microparticles was synergistically effective due to presence of skin components in the formulation and controlled release achieved. This combination is a safe and promising option for the treatment of diabetic wounds requiring long recovery. (C) 2017 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipTUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [1113183]; Ege University Technology Transfer Office [WO/2015/012775 A1]; ANRFrench National Research Agency (ANR) [ANR-10-LABX-33]en_US
dc.description.sponsorshipThis work was supported by TUBITAK 1001 [No: 1113183] and by Ege University Technology Transfer Office for patenting issues [Patent No: WO/2015/012775 A1]. Authors would like to thank FABAL for DSC facilities and Prof. Dr. Mine Hosgor Limoncu (Ege University Faculty of Pharmacy, Department of Microbiology) for sterility evaluation studies and Valerie Nicolas (Plateforme Imagenie Cellulaire, UMS IPSIT (Institut Paris Saclay d'Innovation Therapeutique), Univ. Paris-Sud, Universite Paris-Saclay) for her help with confocal microscopy. Institut Galien Paris-Sud is a member of the Laboratory of Excellence LERMIT supported by a grant from ANR (ANR-10-LABX-33).en_US
dc.identifier.doi10.1016/j.ejpb.2017.04.027en_US
dc.identifier.endpage27en_US
dc.identifier.issn0939-6411
dc.identifier.issn1873-3441
dc.identifier.pmid28461085en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage17en_US
dc.identifier.urihttps://doi.org/10.1016/j.ejpb.2017.04.027
dc.identifier.urihttps://hdl.handle.net/11454/31628
dc.identifier.volume119en_US
dc.identifier.wosWOS:000412041500003en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofEuropean Journal of Pharmaceutics and Biopharmaceuticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCollagenen_US
dc.subjectDermal matrixen_US
dc.subjectLamininen_US
dc.subjectmicroparticlesen_US
dc.subjectResveratrolen_US
dc.subjectWound healingen_US
dc.titleWound healing effects of collagen-laminin dermal matrix impregnated with resveratrol loaded hyaluronic acid-DPPC microparticles in diabetic ratsen_US
dc.typeArticleen_US

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