Purification of alginate and feasible production of monoclonal antibodies by the alginate-immobilized hybridoma cells

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dc.contributor.authorSelimoglu, S. Mert
dc.contributor.authorAyyildiz-Tamis, Duygu
dc.contributor.authorGurhan, Ismet Deliloglu
dc.contributor.authorElibol, Murat
dc.date.accessioned2019-10-27T21:44:31Z
dc.date.available2019-10-27T21:44:31Z
dc.date.issued2012
dc.departmentEge Üniversitesien_US
dc.description.abstractAlginate has an extensive usage in the immobilization of many cell types. Although they have high biocompatibility, commercial alginates contain various degrees of contaminants such as polyphenols, endotoxins and proteins. Thus, these alginates show cytotoxicity against sensitive cell types such as hybridoma cells. In the studies so far, owing to this fact, commercially purchased high-priced ultrapure alginates have been used in the immobilization of hybridoma cells for monoclonal antibody production. However in this study, as a novelty, low-priced commercial alginate was purified, and then the cultivation of alginate-immobilized hybridoma cells was performed for feasible monoclonal antibody production. Low-priced commercial alginate was purified with a profitability ratio of 40%. Then, an optimized immobilization procedure was conducted effectively by using the purified alginate. During more than 25 days of cultivation, serum concentration was kept low, and approximately 2 times greater monoclonal antibody production was achieved, in comparison with its free suspended counterpart. The results showed that the efficiency of monoclonal antibody production via alginate-immobilized hybridoma cultivation can be increased by performing a proved in-house purification method. By shedding light on the efficiency of the in-house purification method, the results also indicated a feasible way of monoclonal antibody production. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.en_US
dc.identifier.doi10.1016/j.jbiosc.2011.09.020en_US
dc.identifier.endpage238en_US
dc.identifier.issn1389-1723
dc.identifier.issn1347-4421
dc.identifier.issue2en_US
dc.identifier.pmid22078279en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage233en_US
dc.identifier.urihttps://doi.org/10.1016/j.jbiosc.2011.09.020
dc.identifier.urihttps://hdl.handle.net/11454/47258
dc.identifier.volume113en_US
dc.identifier.wosWOS:000301688200017en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSoc Bioscience Bioengineering Japanen_US
dc.relation.ispartofJournal of Bioscience and Bioengineeringen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlginateen_US
dc.subjectPurificationen_US
dc.subjectHybridoma cellsen_US
dc.subjectImmobilizationen_US
dc.subjectMonoclonal antibodyen_US
dc.subjectBioprocessen_US
dc.titlePurification of alginate and feasible production of monoclonal antibodies by the alginate-immobilized hybridoma cellsen_US
dc.typeArticleen_US

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