A Ready-to-Use Electrochemical Kit Design for the Diagnosis of Single Nucleotide Polymorphisms

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dc.contributor.authorKayran, Yasin Ugur
dc.contributor.authorOzkan-Ariksoysal, Dilsat
dc.contributor.authorTezcanli, Burcin
dc.contributor.authorKosova, Buket
dc.contributor.authorOzsoz, Mehmet
dc.date.accessioned2019-10-27T22:06:48Z
dc.date.available2019-10-27T22:06:48Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractIn this study, for the first time a model electrochemical kit was constructed for the detection of a functional polymorphism in catechol-O-methyl transferase (COMT) gene which is important for diagnosis of neuropsychiatric disorders as Alzheimer disease. The disposable pencil graphite electrode (PGE) is designed as a kit and the probe DNA covered PGE can detect single nucleotide polymorphisms (SNPs) from real samples based on the guanine oxidation signal even after 5 months of kit preparation (150 days durability).The detection limit (S/N=3) of the biosensor was calculated as 1.18pmol of synthetic target sequence and 6.09x10(5) molecules of real samples in 30min detection time.en_US
dc.description.sponsorshipEge University, Faculty of Pharmacy, Project CoordinationEge University [10 ECZ 022]en_US
dc.description.sponsorshipD.O.A acknowledges technical support from the Pharmaceutical Sciences Research Centre (FABAL) of Ege University, Faculty of Pharmacy. The authors acknowledge the financial support from Ege University, Faculty of Pharmacy, Project Coordination (Project number: 10 ECZ 022).en_US
dc.identifier.doi10.1002/elan.201300386
dc.identifier.endpage2676en_US
dc.identifier.issn1040-0397
dc.identifier.issn1521-4109
dc.identifier.issn1040-0397en_US
dc.identifier.issn1521-4109en_US
dc.identifier.issue12en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage2668en_US
dc.identifier.urihttps://doi.org/10.1002/elan.201300386
dc.identifier.urihttps://hdl.handle.net/11454/48888
dc.identifier.volume25en_US
dc.identifier.wosWOS:000328326100013en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofElectroanalysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectElectrochemical DNA biosensoren_US
dc.subjectPoint-of-care testen_US
dc.subjectReady-to-use kit designen_US
dc.subjectSingle nucleotide polymorphismen_US
dc.subjectCatechol-O-methyl transferase (COMT) Val108en_US
dc.subject158Met polymorphismen_US
dc.titleA Ready-to-Use Electrochemical Kit Design for the Diagnosis of Single Nucleotide Polymorphismsen_US
dc.typeArticleen_US

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