A Ready-to-Use Electrochemical Kit Design for the Diagnosis of Single Nucleotide Polymorphisms
Küçük Resim Yok
Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-V C H Verlag Gmbh
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, for the first time a model electrochemical kit was constructed for the detection of a functional polymorphism in catechol-O-methyl transferase (COMT) gene which is important for diagnosis of neuropsychiatric disorders as Alzheimer disease. The disposable pencil graphite electrode (PGE) is designed as a kit and the probe DNA covered PGE can detect single nucleotide polymorphisms (SNPs) from real samples based on the guanine oxidation signal even after 5 months of kit preparation (150 days durability).The detection limit (S/N=3) of the biosensor was calculated as 1.18pmol of synthetic target sequence and 6.09x10(5) molecules of real samples in 30min detection time.
Açıklama
Anahtar Kelimeler
Electrochemical DNA biosensor, Point-of-care test, Ready-to-use kit design, Single nucleotide polymorphism, Catechol-O-methyl transferase (COMT) Val108, 158Met polymorphism
Kaynak
Electroanalysis
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
25
Sayı
12
