The effects of preconditioning with IFN-?, IL-4, and IL-10 oncostimulatory ligand expressions of mesenchymal stem cells
Küçük Resim Yok
Tarih
2021
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objectives: Mesenchymal stem cells (MSCs) are strong immunomodulatory cells, and co-stimulation may play an im portant role in increasing the effects of MSCs on adaptive immune cells. Preconditioning may add to the effectivenessof MSCs. The aim of this study was to investigate alterations in the costimulatory ligand expressions of MSCs precondi tioned with inflammatory cytokines. Methods: MSCs were preconditioned with interferon gamma (IFN-?), interleukin (IL) 4 (IL-4), and IL-10, and changes inCD80, CD86, CD137L, CD252, CD274, CD275, and human leukocyte antigen (HLA) class I and II expressions were ana lyzed using flow cytometry and quantitative polymerase chain reaction methods. Human acute monocytic leukemiacell line (THP-1) macrophages preconditioned under the same conditions served as a control for comparison. Results: The frequencies of CD80 (p=0.0003), CD86 (p<0.0001), CD137L (p<0.0001), CD252 (p=0.0003), CD274(p=0.0077), CD275 (p<0.0001), and HLA-II (p<0.0001) -positive MSCs was significantly lower than that of the THP-1macrophages with either method, but there was no significant difference in the HLA-I (p=0.1506) cells. Comparison ofthe expression of the costimulatory ligands revealed that the expression of MSCs was significantly lower than that ofTHP-1 cells, and was not affected by cytokine stimuli. Conclusion: The study data indicated that although MSCs are strong immunomodulatory cells, the costimulatory li gand expression required for an effective antigen presentation was extremely low compared with that of professionalantigen presenting cells. In addition, preconditioning with IFN-?, IL-4, and IL-10 failed to increase the expression ofimportant costimulatory ligands, such as CD80 and CD86, in MSCs. The stability of costimulatory ligand expressionsuggests that MSCs may be an effective source for HLA-I-mediated peripheral tolerance.
Açıklama
Anahtar Kelimeler
Kaynak
International Journal of Medical Biochemistry
WoS Q Değeri
Scopus Q Değeri
Cilt
4
Sayı
2
