The effect of different implant biomaterials on the behavior of canine bone marrow stromal cells during their differentiation into osteoblasts

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dc.contributor.authorOzdal-Kurt, F.
dc.contributor.authorTuglu, I.
dc.contributor.authorVatansever, H. S.
dc.contributor.authorTong, S.
dc.contributor.authorSen, B. H.
dc.contributor.authorDeliloglu-Gurhan, S. I.
dc.date.accessioned2019-10-27T22:57:59Z
dc.date.available2019-10-27T22:57:59Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractWe investigated the effects of different implant biomaterials on cultured canine bone marrow stromal cells (BMSC) undergoing differentiation into osteoblasts (dBMSC). BMSC were isolated from canine humerus by marrow aspiration, cultured and differentiated on calcium phosphate scaffold (CPS), hydroxyapatite, hydroxyapatite in gel form and titanium mesh. We used the MTT method to determine the effects of osteogenic media on proliferation. The characteristics of dBMSC were assessed using alizarin red (AR), immunocytochemistry and osteoblastic markers including alkaline phosphatase/von Kossa (ALP/VK), osteocalcin (OC) and osteonectin (ON), and ELISA. The morphology of dBMSC on the biomaterials was investigated using inverted phase contrast microscopy and scanning electron microscopy. We detected expression of ALP/VK, AR, OC and ON by day 7 of culture; expression increased from day 14 until day 21. CPS supported the best adhesion, cell spreading, proliferation and differentiation of BMSCs. The effects of the biomaterials depended on their surface properties. Expression of osteoblastic markers showed that canine dBMSCs became functional osteoblasts. Tissue engineered stem cells can be useful clinically for autologous implants for treating bone wounds.en_US
dc.description.sponsorshipCelal Bayar University Scientific Research Projects CommissionCelal Bayar University [FEF.2003/036]; Scientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [VHAG-2118]en_US
dc.description.sponsorshipThis project was supported by Celal Bayar University Scientific Research Projects Commission (FEF.2003/036) and The Scientific and Technological Research Council of Turkey (VHAG-2118). We thank iNova (Izmir, Turkey) for their kind support.en_US
dc.identifier.doi10.1080/10520295.2016.1183819en_US
dc.identifier.endpage422en_US
dc.identifier.issn1052-0295
dc.identifier.issn1473-7760
dc.identifier.issue6en_US
dc.identifier.pmid27182756en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage412en_US
dc.identifier.urihttps://doi.org/10.1080/10520295.2016.1183819
dc.identifier.urihttps://hdl.handle.net/11454/51395
dc.identifier.volume91en_US
dc.identifier.wosWOS:000382569600004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofBiotechnic & Histochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbiomaterialsen_US
dc.subjectbone marrowen_US
dc.subjectcanineen_US
dc.subjectdifferentiationen_US
dc.subjectimplantsen_US
dc.subjectosteoblasten_US
dc.subjectstromal cellsen_US
dc.subjecttissue engineeringen_US
dc.titleThe effect of different implant biomaterials on the behavior of canine bone marrow stromal cells during their differentiation into osteoblastsen_US
dc.typeArticleen_US

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