Comparative venomics of the vipera ammodytes transcaucasiana and vipera ammodytes montandoni from turkey provides insights into kinship

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dc.contributor.authorHempel B.-F.
dc.contributor.authorDamm M.
dc.contributor.authorGöçmen B.
dc.contributor.authorKaris M.
dc.contributor.authorOguz M.A.
dc.contributor.authorNalbantsoy A.
dc.contributor.authorSüssmuth R.D.
dc.date.accessioned2019-10-27T08:02:54Z
dc.date.available2019-10-27T08:02:54Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractThe Nose-horned Viper (Vipera ammodytes) is one of the most widespread and venomous snakes in Europe, which causes high frequent snakebite accidents. The first comprehensive venom characterization of the regional endemic Transcaucasian Nose-horned Viper (Vipera ammodytes transcaucasiana) and the Transdanubian Sand Viper (Vipera ammodytes montandoni) is reported employing a combination of intact mass profiling and bottom-up proteomics. The bottom-up analysis of both subspecies identified the major snake protein families of viper venoms. Furthermore, intact mass profiling revealed the presence of two tripeptidic metalloprotease inhibitors and their precursors. While previous reports applied multivariate analysis techniques to clarify the taxonomic status of the subspecies, an accurate classification of Vipera ammodytes transcaucasiana is still part of the ongoing research. The comparative analysis of the viper venoms on the proteome level reveals a close relationship between the Vipera ammodytes subspecies, which could be considered to clarify the classification of the Transcaucasian Nose-horned Viper. However, the slightly different ratio of some venom components could be indicating interspecific variations of the two studied subspecies or intraspecies alternations based on small sample size. Additionally, we performed a bioactivity screening with the crude venoms against several human cancerous and non-cancerous cell lines, which showed interesting results against a human breast adenocarcinoma epithelial cell line. Several fractions of Vipera a. transcaucasiana demonstrated a strong cytotoxic effect on triple negative MDA MB 231 breast cancer cells. © 2017 by the authors. Licensee MDPI, Basel, Switzerland.en_US
dc.description.sponsorshipTechnische Universität Berlin Deutsche Forschungsgemeinschaften_US
dc.description.sponsorshipAcknowledgments: We thank Daniel Petras (University of California) and Rashed Al Toma (TU Berlin) for helpful discussion on the manuscript. We acknowledge support by the German Research Foundation and the Open Access Publication Funds of Technische Universität Berlin. --en_US
dc.identifier.doi10.3390/toxins10010023en_US
dc.identifier.issn2072-6651
dc.identifier.issue1en_US
dc.identifier.pmid29301241en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.3390/toxins10010023
dc.identifier.urihttps://hdl.handle.net/11454/25267
dc.identifier.volume10en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMDPI AGen_US
dc.relation.ispartofToxinsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCytotoxicityaen_US
dc.subjectIntact mass profilingen_US
dc.subjectSnake venomicsen_US
dc.subjectTranscaucasian Nose-horned Viperen_US
dc.subjectTransdanubian Sand Viperen_US
dc.subjectTripeptide metalloprotease inhibitoren_US
dc.subjectVipera ammodytesen_US
dc.subjectViperidaeen_US
dc.titleComparative venomics of the vipera ammodytes transcaucasiana and vipera ammodytes montandoni from turkey provides insights into kinshipen_US
dc.typeArticleen_US

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