The detrimental effects of diabetes on pluripotency determined by Klf4, Sox2, c-Myc and Oct4 immunoreactivity in rat testes

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dc.contributor.authorAktug H.
dc.contributor.authorUysal A.
dc.contributor.authorYavasoglu A.
dc.contributor.authorOltulu F.
dc.contributor.authorAkarca S.O.
dc.contributor.authorYilmaz-Dilsiz O.
dc.contributor.authorOktem G.
dc.date.accessioned2019-10-26T21:36:40Z
dc.date.available2019-10-26T21:36:40Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractBackground. Diabetes mellitus (DM) is a multisystem disorder. Type 1 DM can be experimentally induced in rats with streptozotocin (STZ). Diabetic conditions result in testicular oxidative stress and suppressed male reproductive activity as well as decreases in both testicular organ weights and subject weights. Objectives. The purpose of this study was to investigate immunohistochemical differences in testicular tissue due to STZ induced diabetes regarding pluripotency via transcription factors like Klf4, Sox2, c-Myc and Oct4, and to determine weight changes in both the subjects and the testes during the experiment. Material and Methods. Diabetes was induced in male adult rats for this study. A healthy control group and a diabetic group were observed for one month. Blood glucose levels over 250 mg/dL were considered diabetic. Results. On days 0, 3, 15 and 30, the subjects' weights and testicular organ weights were determined and analyzed. The results revealed statistically significant decreases (p < 0.05 and p < 0.001, respectively). Semiquantitative immunohistochemical analyses of Klf4, Sox2, c-Myc and Oct4 were studied in testes paraffin sections via light microscopy. Decreased immunoreactivity of Klf4 was observed in the diabetic group in comparison to the controls. Spermatogonial cells and Sertoli cells snowed increased immunostaining for Sox2 and c-Myc, while decreased immunoreactivity of Oct4 was noted for both spermatogenic and Sertoli cells compared to the control group. Conclusions. This study clearly demonstrated that Klf4, Sox2 and Oct4 immunopositive cells in adult male rat testes manifested sustainable pluripotency and that diabetes has dramatically detrimental effects on this trait. © Copyright by Wroclaw Medical University.en_US
dc.identifier.endpage335en_US
dc.identifier.issn1899-5276
dc.identifier.issue3en_US
dc.identifier.pmid23828673en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage327en_US
dc.identifier.urihttps://hdl.handle.net/11454/18018
dc.identifier.volume22en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofAdvances in Clinical and Experimental Medicineen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectc-Myc transcription factoren_US
dc.subjectDiabetes mellitusen_US
dc.subjectExperimentalen_US
dc.subjectKlf4 proteinen_US
dc.subjectOct4 proteinen_US
dc.subjectRaten_US
dc.subjectSOX2en_US
dc.subjectTestesen_US
dc.titleThe detrimental effects of diabetes on pluripotency determined by Klf4, Sox2, c-Myc and Oct4 immunoreactivity in rat testesen_US
dc.typeConference Objecten_US

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