PHYLOGENETIC DIVERSITY OF MEDITERRANEAN SPONGE MICROBIOME USING DENATURING GRADIENT GEL ELECTROPHORESIS

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dc.contributor.authorKavruk, Ebru
dc.contributor.authorKarabey, Burcin
dc.contributor.authorTemal, Hulya Yilmaz
dc.contributor.authorTuna, Elif Esin Hames
dc.date.accessioned2020-12-01T12:10:01Z
dc.date.available2020-12-01T12:10:01Z
dc.date.issued2019
dc.departmentEge Üniversitesien_US
dc.description.abstractIt has been known that most of the microorganisms that constitute microbial diversity in marine environments have symbiotic relationship with sponges. Metagenomics methods in which the genetic material of the microorganism is used due to the inadequacy of conventional cultivation techniques exposing this diversity took an important place in the recent years. in this study, microbial diversity of four sponge samples; Agelas oroides, Ciocalypta carballoi, Petrosia ficiformis and Sarcotragus foetidus collected from Mediterranean Sea has been analyzed by metagenomics approaches. By using PCR-DGGE (Denaturing Gradient Gel Electrophoresis), a total of 34 16S rDNA-V3 and 10 of ITS1 bands were isolated, sequenced and BLAST analyses were performed to identify the nearest sequence match. According to the results, the bacterial diversity of the four sponges was higher than the fungal diversity. Most common Proteobacteria phylum members were identified in all four sponge samples. This is the first report on the identification of uncultured Roseospirillum sp. from A. oroides, lichen-forming terrestrial uncultured Trichopezizella relicina from P. ficiformis, terrestrial uncultured Cellulomonas sp. and Actinobacterium from C. carballoi. Fungal diversity was determined only in A. oroides and P. ficiformis. Fungal sequences obtained from A. oroides, were found highly similar to uncultured fungal sequences from marine sediment.en_US
dc.description.sponsorshipScientific Research Foundation of Ege UniversityEge University [15 FBE 010]en_US
dc.description.sponsorshipThis study was financially supported by the Scientific Research Foundation of Ege University (Project no. 15 FBE 010). the authors would like to thank Prof. Guven Ozdemir (Department of Biology at Ege University) for their support in DGGE applications.en_US
dc.identifier.endpage10020en_US
dc.identifier.issn1018-4619
dc.identifier.issn1610-2304
dc.identifier.issn1018-4619en_US
dc.identifier.issn1610-2304en_US
dc.identifier.issue12Aen_US
dc.identifier.startpage10011en_US
dc.identifier.urihttps://hdl.handle.net/11454/63630
dc.identifier.volume28en_US
dc.identifier.wosWOS:000501936600043en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherParlar Scientific Publications (P S P)en_US
dc.relation.ispartofFresenius Environmental Bulletinen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMicrobial Diversityen_US
dc.subjectSponge Microbiomeen_US
dc.subjectDenaturing Gradient Gel Electrophoresisen_US
dc.subjectMetagenomicsen_US
dc.titlePHYLOGENETIC DIVERSITY OF MEDITERRANEAN SPONGE MICROBIOME USING DENATURING GRADIENT GEL ELECTROPHORESISen_US
dc.typeArticleen_US

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