PHYLOGENETIC DIVERSITY OF MEDITERRANEAN SPONGE MICROBIOME USING DENATURING GRADIENT GEL ELECTROPHORESIS
Küçük Resim Yok
Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Parlar Scientific Publications (P S P)
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
It has been known that most of the microorganisms that constitute microbial diversity in marine environments have symbiotic relationship with sponges. Metagenomics methods in which the genetic material of the microorganism is used due to the inadequacy of conventional cultivation techniques exposing this diversity took an important place in the recent years. in this study, microbial diversity of four sponge samples; Agelas oroides, Ciocalypta carballoi, Petrosia ficiformis and Sarcotragus foetidus collected from Mediterranean Sea has been analyzed by metagenomics approaches. By using PCR-DGGE (Denaturing Gradient Gel Electrophoresis), a total of 34 16S rDNA-V3 and 10 of ITS1 bands were isolated, sequenced and BLAST analyses were performed to identify the nearest sequence match. According to the results, the bacterial diversity of the four sponges was higher than the fungal diversity. Most common Proteobacteria phylum members were identified in all four sponge samples. This is the first report on the identification of uncultured Roseospirillum sp. from A. oroides, lichen-forming terrestrial uncultured Trichopezizella relicina from P. ficiformis, terrestrial uncultured Cellulomonas sp. and Actinobacterium from C. carballoi. Fungal diversity was determined only in A. oroides and P. ficiformis. Fungal sequences obtained from A. oroides, were found highly similar to uncultured fungal sequences from marine sediment.
Açıklama
Anahtar Kelimeler
Microbial Diversity, Sponge Microbiome, Denaturing Gradient Gel Electrophoresis, Metagenomics
Kaynak
Fresenius Environmental Bulletin
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
28
Sayı
12A
