Effect of Ottoman Viper (Montivipera xanthina (Gray, 1849)) Venom on Various Cancer Cells and on Microorganisms

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dc.contributor.authorYalcin, Husniye Tansel
dc.contributor.authorOzen, Mehmet Ozgun
dc.contributor.authorGocmen, Bayram
dc.contributor.authorNalbantsoy, Ayse
dc.date.accessioned2019-10-27T22:06:21Z
dc.date.available2019-10-27T22:06:21Z
dc.date.issued2014
dc.departmentEge Üniversitesien_US
dc.description.abstractCytotoxic and antimicrobial effects of Montivipera xanthina venom against LNCaP, MCF-7, HT-29, Saos-2, Hep3B, Vero cells and antimicrobial activity against selected bacterial and fungal species: Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, E. coli O157H7, Enterococcus faecalis 29212, Enterococcus faecium DSM 13590, Staphylococcus epidermidis ATCC 12228, S. typhimirium CCM 5445, Proteus vulgaris ATCC 6957 and Candida albicans ATCC 10239 were studied for evaluating the potential medical benefit of this snake venom. Cytotoxicity of venom was determined using MTT assay. Snake venom cytotoxicity was expressed as the venom dose that killed 50 % of the cells (IC50). The antimicrobial activity of venom was studied by minimal inhibitory concentration (MIC) and disc diffusion assay. MIC was determined using broth dilution method. The estimated IC50 values of venom varied from 3.8 to 12.7 or from 1.9 to 7.2 mu g/ml after treatment with crude venom for 24 or 48 h for LNCaP, MCF-7, HT-29 and Saos-2 cells. There was no observable cytotoxic effect on Hep3B and Vero cells. Venom exhibited the most potent activity against C. albicans (MIC, 7.8 mu g/ml and minimal fungicidal concentration, 62.5 mu g/ml) and S. aureus (MIC, 31.25 mu g/ml). This study is the first report showing the potential of M. xanthina venom as an alternative therapeutic approach due to its cytotoxic and antimicrobial effects.en_US
dc.identifier.doi10.1007/s10616-013-9540-zen_US
dc.identifier.endpage94en_US
dc.identifier.issn0920-9069
dc.identifier.issn1573-0778
dc.identifier.issue1en_US
dc.identifier.pmid23381026en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage87en_US
dc.identifier.urihttps://doi.org/10.1007/s10616-013-9540-z
dc.identifier.urihttps://hdl.handle.net/11454/48779
dc.identifier.volume66en_US
dc.identifier.wosWOS:000334174300009en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofCytotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSnake venomen_US
dc.subjectCytotoxicityen_US
dc.subjectAntimicrobial activityen_US
dc.subjectViperen_US
dc.subjectMontivipera xanthinaen_US
dc.titleEffect of Ottoman Viper (Montivipera xanthina (Gray, 1849)) Venom on Various Cancer Cells and on Microorganismsen_US
dc.typeArticleen_US

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