Production of beta-carotene from beet molasses by Blakeslea trispora in stirred-tank and bubble column reactors - Development of a mathematical modeling

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dc.contributor.authorGoksungur, Y
dc.contributor.authorMantzouridou, F
dc.contributor.authorRoukas, T
dc.contributor.authorKotzekidou, P
dc.date.accessioned2019-10-27T18:37:10Z
dc.date.available2019-10-27T18:37:10Z
dc.date.issued2004
dc.departmentEge Üniversitesien_US
dc.description.abstractThe effect of aeration rate and agitation speed on beta-carotene production from molasses by Blakeslea trispora in a stirred-tank fermentor and optimization of the production of the pigment in a bubble column reactor were investigated. In addition, a central composite design was employed to determine the maximum beta-carotene concentration at optimum values for the process variables (aeration rate, sugar concentration, linoleic acid, kerosene). By image analysis of the morphology of the fungus, a quantitative characterization of the hyphae and zygospores formed was obtained. The hyphae were differentiated to intact hyphae, vacuolated hyphae, evacuated cells and degenerated hyphae. An increased proportion of zygospores was correlated to high beta-carotene production. In the stirred-tank fermentor, the highest concentration of the carotenoid pigment (92.0 mg/L) was obtained at an aeration rate of 1.5 vvm and agitation speed of 60 rpm. In the bubble column reactor, the aeration rate and concentration of sugars, linoleic acid, kerosene, and antioxidant significantly affected the production of beta-carotene. In all cases, the fit of the model was found to be good. Aeration rate, sugar concentration, linoleic acid, and kerosene had a strong positive linear effect on beta-carotene concentration. Moreover, the concentration of the pigment was significantly influenced by the negative quadratic effects of the given variables and by their positive or negative interactions. Maximum beta-carotene concentration (360.2 mg/L) was obtained in culture grown in molasses solution containing 5% (w/v) sugar supplemented with linoleic acid (37.59 g/L), kerosene (39.11 g/L), and antioxidant (1.0 g/L).en_US
dc.identifier.doi10.1385/ABAB:112:1:37en_US
dc.identifier.endpage54en_US
dc.identifier.issn0273-2289
dc.identifier.issn1559-0291
dc.identifier.issue1en_US
dc.identifier.pmid14734837en_US
dc.identifier.startpage37en_US
dc.identifier.urihttps://doi.org/10.1385/ABAB:112:1:37
dc.identifier.urihttps://hdl.handle.net/11454/36336
dc.identifier.volume112en_US
dc.identifier.wosWOS:000188289900004en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherHumana Press Incen_US
dc.relation.ispartofApplied Biochemistry and Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbeta-caroteneen_US
dc.subjectbeet molassesen_US
dc.subjectBlakeslea trisporaen_US
dc.subjectstirred-tank fermentoren_US
dc.subjectbubble column reactoren_US
dc.subjectresponse surface methodologyen_US
dc.titleProduction of beta-carotene from beet molasses by Blakeslea trispora in stirred-tank and bubble column reactors - Development of a mathematical modelingen_US
dc.typeArticleen_US

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