Molecular investigation of Rickettsia spp. and Francisella tularensis in ticks from three provinces of Turkey

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dc.contributor.authorDemir, Samiye
dc.contributor.authorAlak, Sedef Erkunt
dc.contributor.authorKoseolu, Ahmet Efe
dc.contributor.authorUn, Cemal
dc.contributor.authorNalcaci, Muhammed
dc.contributor.authorCan, Huseyin
dc.date.accessioned2020-12-01T12:01:19Z
dc.date.available2020-12-01T12:01:19Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractTicks are obligate hematophagous ectoparasites as well as mechanical and biological vectors of a wide variety of microbial pathogens. To date, 19 tick-borne diseases have been reported from Turkey. in this study, ticks collected from Aydin, Izmir and Sanliurfa provinces of Turkey were identified using morphological and molecular methods. After the presence of bacterial DNA was checked, Rickettsia spp. and Francisella tularensis were investigated in bacterial DNA-positive tick specimens by PCR. Furthermore, amplicons belonging to tick specimens and positive bacterial samples were sequenced and processed for BLAST, alignment and phylogenetic analysis. As a result, seven tick species were identified: Rhipicephalus sanguineus, Rh. bursa, Rh. turanicus, Hyalomma marginatum, Hy. aegyptium, Hy. anatolicum and Haemaphysalis erinacei. Fifty-five tick specimens tested positive for bacterial DNA and among them, rickettsial DNA was found in five ticks (infection rate = 9.1%) belonging to Hy. marginatum, Hy. aegyptium, Rh. bursa and Rh. turanicus. of the five Rickettsia-positive ticks, three contained Rickettsia aeschlimannii, one Ri. massiliae and one an unidentified Rickettsia sp. No Francisella tularensis DNA was detected. Sequence analysis of the ompB gene indicated two novel single nucleotide polymorphisms (SNP) in two different Ri. aeschlimannii strains and two novel SNPs as well as a novel insertion (GACGGT) were found in Rickettsia sp. This study indicated the presence of polymorphic Rickettsia species in ticks from Turkey.en_US
dc.identifier.doi10.1007/s10493-020-00498-yen_US
dc.identifier.endpage253en_US
dc.identifier.issn0168-8162
dc.identifier.issn1572-9702
dc.identifier.issue2en_US
dc.identifier.pmid32394036en_US
dc.identifier.scopus2-s2.0-85084519349en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage239en_US
dc.identifier.urihttps://doi.org/10.1007/s10493-020-00498-y
dc.identifier.urihttps://hdl.handle.net/11454/62389
dc.identifier.volume81en_US
dc.identifier.wosWOS:000531743100001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofExperimental and Applied Acarologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectRickettsia aeschlimanniien_US
dc.subjectRickettsia massiliaeen_US
dc.subjectompBen_US
dc.subjectgltAen_US
dc.subjectFrancisella tularensisen_US
dc.subjectHyalomma marginatumen_US
dc.subjectHyalomma aegyptiumen_US
dc.subjectRhipicephalus bursaen_US
dc.subjectRhipicephalus turanicusen_US
dc.titleMolecular investigation of Rickettsia spp. and Francisella tularensis in ticks from three provinces of Turkeyen_US
dc.typeArticleen_US

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