Esophageal Epithelial Resistance and Lower Esophageal Sphincter Muscle Contraction Increase in a Chronic Diabetic Rabbit Model

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dc.contributor.authorCapanoglu, Doga
dc.contributor.authorCoskunsever, Deniz
dc.contributor.authorOlukman, Murat
dc.contributor.authorUlker, Sibel
dc.contributor.authorBor, Serhat
dc.date.accessioned2019-10-27T23:09:22Z
dc.date.available2019-10-27T23:09:22Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractEsophageal motility disorders and possibly gastroesophageal reflux disease are common in patients with diabetes mellitus. We aimed to investigate both the electrophysiological characteristics of the esophageal epithelium and the contractility of the lower esophageal sphincter (LES) muscle in alloxane-induced diabetic rabbits. Electrophysiological properties were measured using an Ussing chamber method. An acid-pepsin model was employed with pH 1.7 or weakly acidic (pH 4) Ringer and/or pepsin. Smooth muscle strips of the LES were mounted in an isolated organ bath. Contractile responses to an electrical field stimulation and cumulative concentrations of acetylcholine were recorded. Contractility of the muscle strips were tested in the presence of Rho-kinase inhibitor (Y-27632) and nonspecific nitric oxide inhibitor (L-NAME). The resistance of diabetic tissue perfused in the pH 1.7 Ringer decreased 17 %; pepsin addition decreased it by 49 %. The same concentrations caused a more distinct loss of resistance in the control tissues (22 and 76 %, p < 0.05). The perfusion of tissues in increased concentrations of luminal and serosal glucose did not change the tissue resistance and voltage. Diabetes significantly increased both the electrical field stimulation and acetylcholine-induced contractions in the LES muscle strips (p < 0.01). Incubation with Y-27632 significantly decreased the acetylcholine-induced contractions in a concentration-dependent manner (p < 0.01). The acid-pepsin model in the diabetic rabbit esophageal tissue had less injury compared with the control. The diabetic rabbit LES muscle had higher contractility, possibly because of the activation of the Rho-Rhokinase pathway. Our results show that in a chronic diabetic rabbit model the esophagus resists reflux by activating mechanisms of mucosal defense and increasing the contractility of the LES.en_US
dc.description.sponsorshipEge University Scientific Research CouncilEge University [2011-TIP-025]en_US
dc.description.sponsorshipThis study has been supported by Ege University Scientific Research Council (project no. 2011-TIP-025).en_US
dc.identifier.doi10.1007/s10620-016-4111-8en_US
dc.identifier.endpage1887en_US
dc.identifier.issn0163-2116
dc.identifier.issn1573-2568
dc.identifier.issue7en_US
dc.identifier.pmid26972084en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1879en_US
dc.identifier.urihttps://doi.org/10.1007/s10620-016-4111-8
dc.identifier.urihttps://hdl.handle.net/11454/52622
dc.identifier.volume61en_US
dc.identifier.wosWOS:000379013300017en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofDigestive Diseases and Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectUssing chambersen_US
dc.subjectEsophagusen_US
dc.subjectDiabetes mellitusen_US
dc.subjectElectrical potential differenceen_US
dc.subjectElectrical field stimulationen_US
dc.subjectEsophageal muscle contractionen_US
dc.titleEsophageal Epithelial Resistance and Lower Esophageal Sphincter Muscle Contraction Increase in a Chronic Diabetic Rabbit Modelen_US
dc.typeArticleen_US

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