A Study on Prophylactic Efficacies of Daptomycin and Vancomycin in a Rat Model of MRSA Infection Secondary to Spinal Implantation
Küçük Resim Yok
Tarih
2013
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Dergi ISSN
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışma deneysel bir enfeksiyon modelinde daptomisin ve vankomisinin profilaktik etkilerini araştırmayı amaçlamaktadır. Yöntem ve Gereç: Bu çalışmada erişkin Winstar cinsi ratların omurgalarına titanyum spinal implantlar yerleştirildikten hemen sonra implant yatağına ve çevre dokuya MRSA ATCC43300 serisi bakteriyel süspansiyon inoküle edildi. Hayvanlar 3 gruba ayrıldı; ilk gruptaki 7 ratantibiyotik almazken ikinci gruptaki 7 rat daptomisin, üçüncü gruptaki 8 rat ise profilaktik olarak vankomisin aldı. mikrobiyolojik ve histolojik değerlendirmeler indüklenmiş enfeksiyonu olan ratlar üzerinde gerçekleştirildi. Bulgular: Derin cerrahi alanların eküvyon kültürlerinde vankomisin hayvanların yarısında (%50) cerrahi alan enfeksiyonu inhibisyonu gösterirken daptomisin kontrol grubu ile karşılaştırıldığında istatistiksel olarak anlamlı (p<0,05) bir şekilde daha fazla rat üzerinde (%71,4) benzer etkiyi gösterdi. İmplant ve kemik kültürleri incelendiğinde ne antibiyotik tedavisi uygulanan gruplarda ne de herhangi bir tedavi almayan kontrol grubunda profilaksi açısından istatistiksel olarak anlamlı bir üstünlük gözlenmedi (p>0,05). Sonuç: Yoğun bakteri inokülasyonu ile enfeksiyonu indüklenmiş spinal implantasyon modelinde daptomisin ve vankomisinin profilaktik etkileri istatistiksel olarak anlamlı bulunmazken daptomisin derin cerrahi alan enfeksiyonu üzerine istatistiksel olarak anlamlı inhibisyon göstermiştir.
The present study aims to investigate the prophylactic effects of daptomycin and vancomycin in an experimental spinal infection induced by spinal implantation. The study involved inoculation of a bacterial suspension prepared with the MRSA ATCC 43300 strain to the implant bed and to the peripheral tissue immediately the implantation of titanium spinal implants to the bone in adult Winstar rats. The animals were divided into three groups; seven rats in the first group received no antibiotics, while the seven rats in group two and eight rats in group three received daptomycin and vancomycin, respectively. Microbiological and histological assessments were carried out on rats with induced infections. In the swab cultures of deep surgical areas, vancomycin demonstrated inhibition of infection of the surgical site in half of animals (50%) whereas daptomycin exhibited the same effect in more animals (71.4%), which was statistically significant compared to the control group (p<0.05). In the implant and bone culture, a statistically significant superiority was not observed in prophylactically treated with either antibiotic and nontreated control group (p>0.05 ). As a result; comparing control group, preventive effects of daptomycin and vancomycin in infection induced model of spinal implantation with high bacteria inoculums were not found statistically significant whereas daptomycin showed statistically significant inhibition on deep surgical area infection.
The present study aims to investigate the prophylactic effects of daptomycin and vancomycin in an experimental spinal infection induced by spinal implantation. The study involved inoculation of a bacterial suspension prepared with the MRSA ATCC 43300 strain to the implant bed and to the peripheral tissue immediately the implantation of titanium spinal implants to the bone in adult Winstar rats. The animals were divided into three groups; seven rats in the first group received no antibiotics, while the seven rats in group two and eight rats in group three received daptomycin and vancomycin, respectively. Microbiological and histological assessments were carried out on rats with induced infections. In the swab cultures of deep surgical areas, vancomycin demonstrated inhibition of infection of the surgical site in half of animals (50%) whereas daptomycin exhibited the same effect in more animals (71.4%), which was statistically significant compared to the control group (p<0.05). In the implant and bone culture, a statistically significant superiority was not observed in prophylactically treated with either antibiotic and nontreated control group (p>0.05 ). As a result; comparing control group, preventive effects of daptomycin and vancomycin in infection induced model of spinal implantation with high bacteria inoculums were not found statistically significant whereas daptomycin showed statistically significant inhibition on deep surgical area infection.
Açıklama
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Nörolojik Bilimler
Kaynak
Journal of Neurological Sciences (Turkish)
WoS Q Değeri
Scopus Q Değeri
Cilt
30
Sayı
4
