In vitro effects of antibiofilm agents and antibiotics oncoagulase-negative staphylococci

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dc.contributor.authorErmertcan, Şafak
dc.contributor.authorLimoncu, Mine Hoşgör
dc.contributor.authorTekintaş, Yamaç
dc.contributor.authorKurutepe, Semra
dc.contributor.authorÖztürk, İsmail
dc.contributor.authorTemel, Aybala
dc.date.accessioned2023-01-12T20:31:56Z
dc.date.available2023-01-12T20:31:56Z
dc.date.issued2020
dc.departmentN/A/Departmenten_US
dc.description.abstractCoagulase negative staphylococci (CoNS) are important nosocomial pathogens that cause biofilm infections. Biofilm provides advantages for microorganisms to resist antibiotics and host immune systems. Considering the increased antibiotic resistance, alternative treatments are needed to combat biofilm infections. In the present study, the effects of antibiotics including gentamicin (GEN), ciprofloxacin, doxycycline (DOX), rifampicin (RIF) and antibiofilm agents including N-acetylcysteine (NAC), ethylenediaminetetraaceticacid (EDTA), nisin (NIS), farnesol (FAR) on clinical CoNS biofilm and IS256, icaA gene expression levels were evaluated. Forty-five CoNS strains were isolated from patients’ catheters, at Manisa Celal Bayar University Hospital. The minimum inhibitory concentrations (MICs) of agents were detected by broth microdilution method with European Committee for Antimicrobial Susceptibility Testing (EUCAST) criteria. The combined effects of agents were investigated by checkerboard method. The antibiofilm effects of combinations were investigated by spectrophotometric microplate method. The effects of combinations on IS256 and icaA gene expressions were evaluated by real-time quantitative reverse-transcriptase PCR. Twenty-four isolates (53.3%) were detected as strong biofilm producer. Biofilm production was inhibited in seven isolates in the presence of EDTA+RIF and NIS+DOX while NIS+GEN combination and RIF inhibited biofilm in six isolates. Nine combinations were found to have synergistic effect against isolate #6 which are resistant to four different antibiotics. The expressions of icaA and IS256 were downregulated in the presence of EDTA, NAC+CIP, NAC+GEN, NIS+GEN, FAR+GEN. Antibiofilm agent/antimicrobial combinations could have promising effects for preventing catheter colonization. The further studies on antibiofilm treatment strategies would be beneficial for decreasing morbidity-mortality rates and healthcare costs caused by biofilms.en_US
dc.identifier.doi10.35333/jrp.2020.241
dc.identifier.endpage832en_US
dc.identifier.issn2630-6344
dc.identifier.issue6en_US
dc.identifier.startpage821en_US
dc.identifier.trdizinid450870en_US
dc.identifier.urihttps://doi.org/10.35333/jrp.2020.241
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/450870
dc.identifier.urihttps://hdl.handle.net/11454/80954
dc.identifier.volume24en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofJournal of research in pharmacy (online)en_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleIn vitro effects of antibiofilm agents and antibiotics oncoagulase-negative staphylococcien_US
dc.typeArticleen_US

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