Kersetin ve klorheksidin yüklü PLGA formülasyonlarının hazırlanması ve periodontal hastalıkların tedavisi için prototip ürün geliştirilmesi
Küçük Resim Yok
Tarih
2024
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Ege Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Periodontal hastalıklar, diş eti oluğu içerisinde yerleşmiş olan mikrobiyal dental plak ile konak cevapları arasındaki etkileşimler sonucu ortaya çıkan enflamatuvar hastalıklardır. Projemizde periodontitis tedavisinde lokal olarak uygulanacak kersetin ve klorheksidin yüklü Poli(D,L-laktik-ko-glikolik asit) nanopartikül sisteminin prototip ürününün geliştirilmesi, in vitro ve in vivo olarak antimikrobiyal ve antienflamatuvar çift yönlü etkinliğinin incelenmesi hedeflenmektedir. Bu amaçla, geniş spektrumlu antimikrobiyal özellikleri nedeniyle ağız bakım ürünlerinde yaygın olarak kullanılan klorheksidin (CHX) ile doğal bir ürün olan antibakteriyel etkinliğinin yanısıra, antienflamatuvar ve yara iyileşmesini hızlandırma özellikleri bilinen kersetinin (QUE) farklı oranlarda (%1,5, 5 ve 15) tekli ve ikili sistemleri Poli(D,L-laktik-ko-glikolik asit) nanopartiküllerine (PLGA NP) yüksek verimle yüklenerek yapısal, morfolojik ve termal özellikleri karakterize edilmiştir. QUE ve CHX yüklü nanopartiküllerin yapay tükürük ortamında in-vitro salım çalışmaları gerçekleştirilerek, yüksek basınçlı sıvı kromatografisi (HPLC) ile CHX ve QUE'in miktarları tayin edilmiştir. CHX ve QUE etkin maddeleri için kısmi validasyon çalışması gerçekleştirilmiş olup sonuçlar belirlenen kriterlere uygunluk sağlamıştır. Sentezlenen CHX/ QUE etkin maddeleri yüklü PLGA NP Dinamik Işık Saçılımı (DLS) sonuçları incelendiğinde, boyutların 60-180 nm aralığında değiştiği görülmüştür. +4oC koşulunda yapılan stabilite testi verileri değerlendirildiğinde, her iki etkin maddenin de stabil olduğu sonucuna varılmıştır. Yapay tükürük ortamında QUE etkin maddesi tek başına yüklendiğinde 168 saat salım gösterirken, CHX/ QUE etkin maddeleri ikili olarak yüklendiğinde 240. Saat sonunda bile %20 lerde etkinlik gösterdiği bulunmuştur. CHX etkin maddesi ise tekli ve ikili her iki sistem için 240. saattin sonunda %70 in üstünde etkinlik gösterdiği görülmektedir. Ayrıca Albino Wistar sıçanların üst çenelerindeki ikinci azı dişlerinin çevresine bağlanan ipek dikiş materyali ile 21. gün sonunda deneysel periodontitis hastalık modeli geliştirildi. Bu proje çalışması TUA-2020-22540 nolu Ege Üniversitesi Bilimsel Araştırma Projesi ile desteklenmiştir. Ege Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü'ne teşekkür ederiz.
Periodontal diseases are inflammatory diseases that occur as a result of interactions between microbial dental plaque settled in the gingival groove and host responses. Our project aims to develop a prototype product of the quercetin and chlorhexidine loaded PLGA system to be applied locally in the treatment of periodontitis and to examine its antimicrobial and anti-inflammatory bidirectional effectiveness in vitro and in vivo. For this purpose, chlorhexidine (CHX), which is widely used in oral care products due to its broad-spectrum antimicrobial properties, and quercetin (QUE), which is a natural product known for its antibacterial activity as well as its anti-inflammatory and wound healing accelerating properties, were used in different amounts (1.5, 5 and 15%). The CHX and QUE active substances were loaded single or binary into Poly(D,L-lactic-coglycolic acid) nanoparticles (PLGA NP) with high efficiency, and their structural, morphological and thermal properties were characterized. In-vitro release studies of CHX/ QUE loaded nanoparticle were carried out in artificial saliva environment, and the amounts of CHX and QUE were determined by high-pressure liquid chromatography (HPLC). A partial validation study was carried out for the active substances CHX and QUE, and the results met the specified criteria. When the Dynamic Light Scattering (DLS) results of PLGA NPs loaded with the synthesized CHX/ QUE active ingredients were examined, it was seen that the dimensions varied in the range of 100-150 nm. When the stability test data performed under +4 oC conditions were evaluated, it was concluded that both active substances were stable. While the QUE active ingredient showed a release of 168 hours when loaded alone in the artificial saliva environment, it was found that when the CHX/QUE active ingredients were loaded dual, it showed an effectiveness of 20% even at the end of the 240th hour. As for the active ingredient CHX, it is observed that it is more than 70% effective even at the end of the 240th hour for both single and dual systems. In addition, an experimental periodontitis disease model was developed at the end of the 21st day by silk suture materials tied around the second molars in the upper jaw of Albino Wistar rats. This project work was supported by the Ege University Scientific Research Project numbered TUA-2020-22540. We would like to thank Ege University Scientific Research Projects Coordination Office.
Periodontal diseases are inflammatory diseases that occur as a result of interactions between microbial dental plaque settled in the gingival groove and host responses. Our project aims to develop a prototype product of the quercetin and chlorhexidine loaded PLGA system to be applied locally in the treatment of periodontitis and to examine its antimicrobial and anti-inflammatory bidirectional effectiveness in vitro and in vivo. For this purpose, chlorhexidine (CHX), which is widely used in oral care products due to its broad-spectrum antimicrobial properties, and quercetin (QUE), which is a natural product known for its antibacterial activity as well as its anti-inflammatory and wound healing accelerating properties, were used in different amounts (1.5, 5 and 15%). The CHX and QUE active substances were loaded single or binary into Poly(D,L-lactic-coglycolic acid) nanoparticles (PLGA NP) with high efficiency, and their structural, morphological and thermal properties were characterized. In-vitro release studies of CHX/ QUE loaded nanoparticle were carried out in artificial saliva environment, and the amounts of CHX and QUE were determined by high-pressure liquid chromatography (HPLC). A partial validation study was carried out for the active substances CHX and QUE, and the results met the specified criteria. When the Dynamic Light Scattering (DLS) results of PLGA NPs loaded with the synthesized CHX/ QUE active ingredients were examined, it was seen that the dimensions varied in the range of 100-150 nm. When the stability test data performed under +4 oC conditions were evaluated, it was concluded that both active substances were stable. While the QUE active ingredient showed a release of 168 hours when loaded alone in the artificial saliva environment, it was found that when the CHX/QUE active ingredients were loaded dual, it showed an effectiveness of 20% even at the end of the 240th hour. As for the active ingredient CHX, it is observed that it is more than 70% effective even at the end of the 240th hour for both single and dual systems. In addition, an experimental periodontitis disease model was developed at the end of the 21st day by silk suture materials tied around the second molars in the upper jaw of Albino Wistar rats. This project work was supported by the Ege University Scientific Research Project numbered TUA-2020-22540. We would like to thank Ege University Scientific Research Projects Coordination Office.
Açıklama
Anahtar Kelimeler
Kersetin, Klorheksidin, Periodontal Hastalıklar, Poli(D, L-laktik-koglikolik asit)., Quercetin, Chlorhexidine, Periodontal Diseases, Poly(D, L-lactic-coglycolic acid).
