Kronik C hepatitinde HCV genotiplerinin dağılımı
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Tarih
2001
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info:eu-repo/semantics/openAccess
Özet
Bu çalışmada 1997-1999 yılları arasında Ege Üniversitesi Tıp Fakültesi Hastanesi'ne başvuran kronik hepatit C virüs (HCV) enfeksiyonu tanısı almış 170 hastada HCV genotipleri araştırılmıştır. HCV genotiplerini belirlemek için HCV-RNA tarama testi olarak kullandığımız RT-PCR (HCV Amplicor Test) yöntemi ile elde edilen ürünler; line probe assay (LiRA) testi ile genotiplendirilmiştir. Bu yöntemle genotiplendirilemeyen örnek olmayıp, genotip 1b en sık saptanan genotip olarak bulunmuştur (%81.2). Bunu genotip 1a (%10) izlemektedir. Ayrıca genotip 2a/2c (%1.8), 2b (%0.6), 3a (%0.6), 4 (%0.6) ve 4c/4d (%0.6) saptanmış olup hastaların %4.7'sinde birden fazla genotip belirlenmiştir. Örneklerin büyük çoğunluğu Gastroenteroloji kliniği hastalarına aittir ve yaş gruplarına göre genotiplerin dağılımında farklılık saptanmamıştır.
In this study, hepatitis C virus (HCV) genotypes of 170 chronic hepatitis C patients, admitted to Ege University Hospital between 1997-1999;. were determined. Reverse transcriptase PCR (RT-PCR, HCV Amplicor Test) method was used for initial screening to identify arriplicons 'of HCV-RNA and these products were genotyped with line probe assay (LİPA). All the samples were genotyped, and genotype 1b was found to be the most predominant type (81.2%), followed by genotype 1a (10%). Genotype 2a/2c (1.8%), 2b (0.6%), 3a (0.6%), 4 (0.6%) and 4c/4d (0.6%) were the other identified types, and in 4.7% of the patients multiple genotypes were detected. The majority of'the samples were obtained from the patients of Gastroenterology clinics, and there was no significant difference between the age groups and genotype distribution.
In this study, hepatitis C virus (HCV) genotypes of 170 chronic hepatitis C patients, admitted to Ege University Hospital between 1997-1999;. were determined. Reverse transcriptase PCR (RT-PCR, HCV Amplicor Test) method was used for initial screening to identify arriplicons 'of HCV-RNA and these products were genotyped with line probe assay (LİPA). All the samples were genotyped, and genotype 1b was found to be the most predominant type (81.2%), followed by genotype 1a (10%). Genotype 2a/2c (1.8%), 2b (0.6%), 3a (0.6%), 4 (0.6%) and 4c/4d (0.6%) were the other identified types, and in 4.7% of the patients multiple genotypes were detected. The majority of'the samples were obtained from the patients of Gastroenterology clinics, and there was no significant difference between the age groups and genotype distribution.
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Cilt
35
Sayı
3
