Momordica Charantia L. Meyveleri Kullanılarak Krem Hazırlanması, Kalite Kontrolü ve In Vitro Yara İyi Edici Etkisinin Araştırılması
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Tarih
2019
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Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışma, halk arasında geleneksel olarak dahilen ve haricen yara iyileştirici olarak kullanılan kudret narı (Momordica charantia)-zeytinyağı karışımından yara iyi edici prototip krem formunun hazırlanması, hazırlanan krem formunun standardizasyonu ve kalite kontrol çalışmaları ile yara iyileştirici etkisinin belirlenmesi için in vitro hücre kültürü yöntemi kullanılarak araştırılması amacıyla dizayn edilmiştir. Çalışma kapsamında öncelikle kudret narı zeytinyağı karışımı hazırlanmıştır. Hazırlanan krem formunun total flavonoid içerikleri spektrofotometrik ve yüksek basınçlı sıvı kromatografi yöntemleri kullanılarak belirlenmiştir. Hazırlanan Kudret narı krem formunun invitro kalite kontrol çalışmalarında total flavonoid miktarı, pH, vizkozite ve stabilite tayinleri ile biyolojik aktivitelerinin belirlenmesi kapsamında, BJ ve HaCaT hücreleri ile gerçekleştirilen in vitro hücre migrasyonu çalışmaları ile yara iyileştirici etkileri belirlenmiştir. Kudret narı – zeytinyağı maseratı ve farmasötik kalitede yardımcı maddeler kullanılarak hazırlanan laboratuvar ölçekli krem formu total flavonoid içeriği üzerinden standardize edilmiş, yapılan 40 °C % 60 nemde yürütülen çalışmalarda ürünün stabil olduğu ve in vitro yara iyileştirme modelinde BJ ve HaCaT hücrelerinde artan dozlarla (1,10,30) birlikte kontrol grubuna karşılık hücre migrasyonunun arttığı gözlenmiştir
This study was designed for preparation of wound-healing cream form from the mixture of olive oil and Momordica charantia, used as wound healing agent. We also aimed to employ the standardization and quality control studies of prepared cream form and to investigate its wound healing effect using in vitro cell culture method. Within the scope of this study, first a mixture of olive oil and Momordica charantia was prepared. Total flavonoid contents of prepared cream form were determined by using spectrophotometry and high pressure liquid chromatography. Quality control studies of this cream were studied in vitro to determine the total amount of flavonoid, pH, viscosity and stability. Biological activities of prepared cream form were studied to determine the cell migration and wound healing effect on BJ and HaCaT cells by in vitro cell culture. The laboratory scale cream form, prepared using the mixture of olive oil and Momordica charantia together with pharmaceutical quality excipients, was standardized on the total flavonoid content. The product was found to be stable at 40°C with 60% humidity. In in vitro wound healing models of BJ and HaCaT cells with increasing doses of cream form, cell migration was observed to increase, compared to control group.
This study was designed for preparation of wound-healing cream form from the mixture of olive oil and Momordica charantia, used as wound healing agent. We also aimed to employ the standardization and quality control studies of prepared cream form and to investigate its wound healing effect using in vitro cell culture method. Within the scope of this study, first a mixture of olive oil and Momordica charantia was prepared. Total flavonoid contents of prepared cream form were determined by using spectrophotometry and high pressure liquid chromatography. Quality control studies of this cream were studied in vitro to determine the total amount of flavonoid, pH, viscosity and stability. Biological activities of prepared cream form were studied to determine the cell migration and wound healing effect on BJ and HaCaT cells by in vitro cell culture. The laboratory scale cream form, prepared using the mixture of olive oil and Momordica charantia together with pharmaceutical quality excipients, was standardized on the total flavonoid content. The product was found to be stable at 40°C with 60% humidity. In in vitro wound healing models of BJ and HaCaT cells with increasing doses of cream form, cell migration was observed to increase, compared to control group.
Açıklama
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Kaynak
Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi
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Scopus Q Değeri
Cilt
12
Sayı
1
