Analysis of Vitamin-A In Eggs By High-Pressure Liquid-Chromatography

A high speed liquid chromatographic method was described for the rapid determination of vitamin A in eggs. After saponification and extraction, the sample was separated on a reversed-phase column (C18) with acetonitrile - methylene-chloride - methanol as the mobile phase. Quantitative determination was accomplished by monitoring the ultraviolet absorbance of retinol at 280 nm. Recovery of vitamin A was 97.1 %.