Molecular evaluation of cytokeratin 20 mRNA expression of transitional cell carcinoma cases
Küçük Resim Yok
Tarih
2011
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Sitokeratinler 20 polipeptitten meydana gelen ve epitel hücrelerinin intermediyal filamentlerinden şekil alan multigenik proteinlerdir. Sitokeratin 20 mRNA ekspresyon seviyesinin mesane kanserinin erken tanısında kantitatif farkı çok önemlidir.Bu çalışmada tümör prognozu ve rekürrensinde Sitokeratin 20 mRNA ekspresyonunun güvenirliliğinin araştırılması amaçlanmıştır. Yöntem ve Gereç: Transizyonel hücre karsinomu tanılı 34 olgu [Ta(2),T1(19),T2(11),T3(1),T4(1)] ve kontrol olarak 10 sağlıklı gönüllü çalışmaya alınmıştır.Sitokeratin 20 mRNA ekspresyonunun kantifikasyonu Revers Transkriptaz PCR ile gerçekleştirilmiştir. Bulgular: Sitokeratin 20 mRNA ekspresyonunun ortalama rölatif oranı transizyonel hücre karsinomu tanılı 34 olguda 11.91±50.01 ve kontrol grubunda 0.07±0.12 olarak bulundu. En yüksek Sitokeratin 20 mRNA ekspresyon değeri 25.62 olarak T1/G2 grubunda görüldü.Sitokeratin 20 mRNA ekspresyonu ve tümör derecesi arasında anlamlı bir korelasyon bulunmadı.Pozitif ve negatif olgu sonuçları cut-off,spesifite ve sensitivite, metodun pozitif,negatif ve total tanı değerleri sırası ile %69.56, %67.65, %85.19, %59.26, %78.00 olarak hesaplandı. ROC (Receiver Operating Characteristic) eğrisi altındaki alan 0.917 (p<0, 0001) olarak bulundu.Sensitivitesi %82,4 ve spesifitesi %81,2 olan Sitokeratin 20 mRNA ekspresyonunun cut-off değeri 0,1224 olarak hesaplandı. Sonuç: Artmış Sitokeratin 20 mRNA ekspresyonu ile birlikte tümörün patolojik sınıfı agresif tümör davranışının bir indikatörü olarak gözlenmiş ve bu hastaların daha yakından izlenmesi gerekliliğini ortaya koymuştur.İdrar örneklerindeki Sitokeratin 20 mRNA ekspresyonunun değerlendirilmesinin, papilloma veya tümörlü hastaların tanısında yardımcı olabileceğini düşünmekteyiz.
Aim: Cytokeratins are multigenic proteins which consist of twenty polypeptides and are formed by intermediate filament of epithelial cells. The quantitatively stated difference in the level of Cytokeratin 20 mRNA expression is considerable in early detection of bladder carcinoma. This study aimed to evaluate the value of Cytokeratin 20 mRNA expression in tumor prognosis and recurrence. Material and Methods: Thirty-four cases [Ta(2),T1(19),T2(11),T3(1),T4(1)] diagnosed as transitional cell carcinoma and 10 healthy volunteers as control group were recruited. The quantification of Cytokeratin 20 mRNA expression was performed by Reverse Transcriptase PCR. Results: The average relative ratio of Cytokeratin 20 mRNA expression was 11.91±50.01 in 34 cases with transitional cell carcinoma and 0.07±0.12 in control cases. The highest expression of Cytokeratin 20 mRNA was found in T1/G2 as 25.62. No significant correlation was found between the Cytokeratin 20 mRNA expression and tumor grading. The results were determined according to the cut-off, specificity and sensitivity were 69.56% and 67.65%, respectively with positive, negative and total diagnostic values as; 85.19%,59.26% and 78.00%. The area under the Receiver Operating Characteristic curve was 0.917 (p<0.0001). The cut-off value of Cytokeratin 20 mRNA expression that had the sensitivity (82.4%) and the specificity (81.2%) was determined as 0.1224. Conclusion: We suggest that the combination of increased Cytokeratin 20 mRNA expression and pathological tumor classification is an indicator of more aggressive tumor behavior and the cases should be monitored more closely. The evaluation of Cytokeratin 20 mRNA expression in urine samples will be quite useful in the diagnosis of increased Cytokeratin 20 mRNA expression in the cases with papilloma or tumor.
Aim: Cytokeratins are multigenic proteins which consist of twenty polypeptides and are formed by intermediate filament of epithelial cells. The quantitatively stated difference in the level of Cytokeratin 20 mRNA expression is considerable in early detection of bladder carcinoma. This study aimed to evaluate the value of Cytokeratin 20 mRNA expression in tumor prognosis and recurrence. Material and Methods: Thirty-four cases [Ta(2),T1(19),T2(11),T3(1),T4(1)] diagnosed as transitional cell carcinoma and 10 healthy volunteers as control group were recruited. The quantification of Cytokeratin 20 mRNA expression was performed by Reverse Transcriptase PCR. Results: The average relative ratio of Cytokeratin 20 mRNA expression was 11.91±50.01 in 34 cases with transitional cell carcinoma and 0.07±0.12 in control cases. The highest expression of Cytokeratin 20 mRNA was found in T1/G2 as 25.62. No significant correlation was found between the Cytokeratin 20 mRNA expression and tumor grading. The results were determined according to the cut-off, specificity and sensitivity were 69.56% and 67.65%, respectively with positive, negative and total diagnostic values as; 85.19%,59.26% and 78.00%. The area under the Receiver Operating Characteristic curve was 0.917 (p<0.0001). The cut-off value of Cytokeratin 20 mRNA expression that had the sensitivity (82.4%) and the specificity (81.2%) was determined as 0.1224. Conclusion: We suggest that the combination of increased Cytokeratin 20 mRNA expression and pathological tumor classification is an indicator of more aggressive tumor behavior and the cases should be monitored more closely. The evaluation of Cytokeratin 20 mRNA expression in urine samples will be quite useful in the diagnosis of increased Cytokeratin 20 mRNA expression in the cases with papilloma or tumor.
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Cerrahi
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Ege Tıp Dergisi
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Scopus Q Değeri
Cilt
50
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2
