Pits ve fissür örtücü uygulamalarında, lazer ve konvansiyonel pürüzlendirme yöntemlerinin, bağ dayanımı ve mikrosızıntı üzerindeki etkilerinin in-vitro koşullarda karşılaştırmalı olarak araştırılması
Küçük Resim Yok
Tarih
2015
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Ege Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmanın amacı; Er:YAG lazer ve konvansiyonel pürüzlendirme sonrası bonding ajanı uygulayarak veya uygulamayarak yapılan rezin ve cam polialkenoat esaslı fissür örtücülerin mikrosızıntı ve bağ dayanımının in-vitro koşullarda karşılaştırmalı olarak araştırılmasıdır. Çalışmada 6 aydan kısa süre önce çekilmiş, çürüksüz 299 adet üçüncü molar diş kullanıldı. Dişler mikrosızıntı için n=16, bağ dayanımı için n=7 olacak şekilde 13 gruba ayrıldı: Lazer+Clinpro® (G1), lazer+asit+Clinpro® (G2), lazer+asit+bond+Clinpro® (G3), lazer+bond+Clinpro® (G4), asit+Clinpro® (G5), asit+bond+Clinpro® (G6), bond+Clinpro® (G7), lazer+Fuji Triage® (G8), lazer+bond+Fuji Triage® (G9), lazer+conditioner+Fuji Triage® (G10), lazer+conditioner+bond+Fuji Triage® (G11), conditioner+Fuji Triage® (G12) ve doğrudan Fuji Triage® (G13). Termal siklus (1000X, 5-55°C) uygulanan dişlerin apeksleri kompozit ile tıkandıktan sonra fissür örtücü marjinlerinin çevresinde 1 mm açıklık kalacak şekilde dişler tırnak cilası ile 2 kat kaplandı. 24 saat %0,5'lik bazik fuksinde bekletilen dişler akan musluk suyu altında yıkandıktan sonra akril bloklara gömüldü. Akril bloklar içindeki dişler, elmas bıçak (Isomet, Buehler®; Lake Bluff, IL, ABD) ile bukkolingual olarak birbirine paralel 3 kısımdan kesilerek ışık mikroskobu altında (Leicamicrosystems® stereo microscope, Ltd.Stereo and microscope systems; Heerburg, İsviçre) değerlendirildi. Bağ dayanımı testinde dişler, vestibül ya da lingual mine yüzeyleri açıkta kalacak şekilde akril bloklara gömüldü. Gruplara göre yüzey hazırlıkları yapıldıktan sonra, özel olarak hazırlanan pleksiglass kalıp yardımıyla fissür örtücüler 2,5mm x 3,5mm boyutlarında hazırlandı. 24 saat distile suda bekletilen örneklerin makaslama bağ dayanımı testi için üniversal test cihazı (Shimadzu KgN50®. Shimadzu Corp. Kyoto, Japonya) kullanıldı. Örnekler kırılana kadar 0,5 mm/dk hızda kuvvet uygulandı. Örnek yüzeyleri ışık mikroskobu altında (Leicamicrosystems® stereo microscope, Ltd.Stereo and microscope systems; Heerburg, İsviçre) x10 büyütmede incelendi ve gruplardan seçilen örnekler taramalı elektron mikroskobu (SEM) (Jeol JSM® 5200, Kyoto, Japonya) ile değerlendirildi. Elde edilen verilerin istatistiksel analizinde SPSS 19.0 (SPSS Inc®. Chicago, IL, ABD) istatistik programı kullanılarak; mikrosızıntı için Kruskal-Wallis ve Mann Whitney U testi (p<0,05), bağ dayanımı için Shapiro-Wilk, Oneway Anova, Levene, Welch ve Dunnett T3 testi yapıldı (p<0,05). Mikrosızıntı testi bulgularına göre; lazer+asit+Clinpro®, lazer+asit+bond+Clinpro®, lazer+bond+Clinpro® ve asit+Clinpro® grupları diğer gruplara göre istatistiksel olarak anlamlı ölçüde daha düşük skorlara sahip bulundu (p<0,05). Bağ dayanımı bulgularına göre ise; asit+Clinpro®, asit+bond+Clinpro®, lazer+asit+Clinpro® grupları diğer gruplara kıyasla anlamlı ölçüde daha yüksek bağ dayanımı gösterdi (p<0,05). Sonuç olarak; mikrosızıntı ve bağ dayanımı bulguları birlikte değerlendirildiğinde rezin esaslı fissür örtücüler cam polialkenoat esaslılara göre anlamlı ölçüde daha düşük sızıntı ve daha yüksek bağ dayanımı göstermiş olup, bu bulgular ışığında lazer ve/veya bonding ajanı kullanımının, asit ile pürüzlendirmeye duyulan ihtiyacı gideremediği, geleneksel pürüzlendirme yönteminin hala en iyi sonuçlara sahip olduğu, daha fazla aşamalı tekniklerin tedavi süresini uzatması sebebiyle klinik uygulamalarda önerilmemesi gerektiği sonuçlarına ulaşıldı
The aim of this study was to comparatively research the bond strength and microleakage of resin and polyalkenoate based fissure sealants made by applying or not applying the bonding agent following Er:YAG laser and conventional etching. In the study, 299 intact third molar teeth extracted in 6 months were used. Teeth were seperated into 13 groups, in which n=16 for microleakage and n=7 for bond strength: Laser+Clinpro® (G1), laser+acid+Clinpro® (G2), laser+acid+bond+Clinpro® (G3), laser+bond+Clinpro® (G4), acid+Clinpro® (G5), acid+bond+Clinpro® (G6), bond+Clinpro® (G7), laser+Fuji Triage® (G8), laser+bond+Fuji Triage® (G9), laser+conditioner+Fuji Triage® (G10), laser+conditioner+bond+Fuji Triage® (G11), conditioner+Fuji Triage® (G12) and direct Fuji Triage® (G13). The teeth were thermocycled (1000X, 5-55°C) and were covered with two layers of nail polish, leaving a 1mm gap around their fissure sealant margines after their apices were blocked by composite. The teeth were kept in % 0.5 basic fuchsine for 24 hours, washed under tap water and embedded in acryl blocks. The teeth in acryl blocks, were cut buccolingually through 3 parallel parts by a diamond knife (Isomet, Buehler®; Lake Bluff, IL, USA) and were evaluated under a stereomicroscope (Leicamicrosystems® stereo microscope, Ltd.Stereo and microscope systems; Heerburg, Switzerland). In the bond strength test, the teeth were embedded into acryl blocks, leaving their vestibule or lingual enamel surfaces open. After the surface treatment was complete based on the groups, 2,5 mmx3,5 mm fissure sealants were prepared with the help of a specially prepared plexiglass mould. Universal testing device (Shimadzu KgN50®. Shimadzu Corp. Kyoto, Japan) was used for the shear bond strength test of the specimens which were kept in distilled water for 24 hours. 0,5 mm/min force had been applied until the specimens were broken. Surfaces of the specimens were examined under a stereomicroscope (Leicamicrosystems® stereo microscope, Ltd.Stereo and microscope systems; Heerburg, Switzerland) by enlarging them x10 and the specimens selected from the groups were evaluated by a scanning electron microscope (SEM) (Jeol JSM® 5200, Kyoto, Japan). The acquired data was analyzed by SPSS 19.0 (SPSS Inc®. Chicago, IL, USA) statistics software; for microleakage Kruskal-Wallis and Mann Whitney U test (p<0,05) and for bond strength Shapiro-Wilk, Oneway Anova, Levene, Welch and Dunnett T3 test was performed(p<0,05). According to microleakage test findings; laser+acid+Clinpro®, laser+acid+bond+Clinpro®, laser+bond+Clinpro® and acid+Clinpro® groups were found to have less statistically significant scores compared to the other groups (p<0,05). According to the bond strength findings; acid+Clinpro®, acid+bond+Clinpro®, laser+acid+Clinpro® groups had a more statistically significant bond strength compared to the other groups (p<0,05). As a result; when microleakage and bond strength findings were evaluated together, resin based fissure sealants had significantly lower leakage compared to glass polyalkeonate based sealants and under the light of these findings it was found that laser and/or bonding agent usage does not suffice to meet the need of acid etching and conventional etching method still has better results and methods with more stages prolong the duration of the treatment, thus should not be suggested for clinical applications.
The aim of this study was to comparatively research the bond strength and microleakage of resin and polyalkenoate based fissure sealants made by applying or not applying the bonding agent following Er:YAG laser and conventional etching. In the study, 299 intact third molar teeth extracted in 6 months were used. Teeth were seperated into 13 groups, in which n=16 for microleakage and n=7 for bond strength: Laser+Clinpro® (G1), laser+acid+Clinpro® (G2), laser+acid+bond+Clinpro® (G3), laser+bond+Clinpro® (G4), acid+Clinpro® (G5), acid+bond+Clinpro® (G6), bond+Clinpro® (G7), laser+Fuji Triage® (G8), laser+bond+Fuji Triage® (G9), laser+conditioner+Fuji Triage® (G10), laser+conditioner+bond+Fuji Triage® (G11), conditioner+Fuji Triage® (G12) and direct Fuji Triage® (G13). The teeth were thermocycled (1000X, 5-55°C) and were covered with two layers of nail polish, leaving a 1mm gap around their fissure sealant margines after their apices were blocked by composite. The teeth were kept in % 0.5 basic fuchsine for 24 hours, washed under tap water and embedded in acryl blocks. The teeth in acryl blocks, were cut buccolingually through 3 parallel parts by a diamond knife (Isomet, Buehler®; Lake Bluff, IL, USA) and were evaluated under a stereomicroscope (Leicamicrosystems® stereo microscope, Ltd.Stereo and microscope systems; Heerburg, Switzerland). In the bond strength test, the teeth were embedded into acryl blocks, leaving their vestibule or lingual enamel surfaces open. After the surface treatment was complete based on the groups, 2,5 mmx3,5 mm fissure sealants were prepared with the help of a specially prepared plexiglass mould. Universal testing device (Shimadzu KgN50®. Shimadzu Corp. Kyoto, Japan) was used for the shear bond strength test of the specimens which were kept in distilled water for 24 hours. 0,5 mm/min force had been applied until the specimens were broken. Surfaces of the specimens were examined under a stereomicroscope (Leicamicrosystems® stereo microscope, Ltd.Stereo and microscope systems; Heerburg, Switzerland) by enlarging them x10 and the specimens selected from the groups were evaluated by a scanning electron microscope (SEM) (Jeol JSM® 5200, Kyoto, Japan). The acquired data was analyzed by SPSS 19.0 (SPSS Inc®. Chicago, IL, USA) statistics software; for microleakage Kruskal-Wallis and Mann Whitney U test (p<0,05) and for bond strength Shapiro-Wilk, Oneway Anova, Levene, Welch and Dunnett T3 test was performed(p<0,05). According to microleakage test findings; laser+acid+Clinpro®, laser+acid+bond+Clinpro®, laser+bond+Clinpro® and acid+Clinpro® groups were found to have less statistically significant scores compared to the other groups (p<0,05). According to the bond strength findings; acid+Clinpro®, acid+bond+Clinpro®, laser+acid+Clinpro® groups had a more statistically significant bond strength compared to the other groups (p<0,05). As a result; when microleakage and bond strength findings were evaluated together, resin based fissure sealants had significantly lower leakage compared to glass polyalkeonate based sealants and under the light of these findings it was found that laser and/or bonding agent usage does not suffice to meet the need of acid etching and conventional etching method still has better results and methods with more stages prolong the duration of the treatment, thus should not be suggested for clinical applications.
Açıklama
Anahtar Kelimeler
Diş Hekimliği, Dentistry
