Enhanced Anti-cancer Potency Using a Combination of Oleanolic Acid and Maslinic Acid to Control Treatment Resistance in Breast Cancer

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dc.authoridsogutlu, fatma/0000-0002-1210-7660
dc.authorscopusid22952992800
dc.authorscopusid57203588534
dc.authorscopusid57203689522
dc.authorscopusid57216431373
dc.authorscopusid6603599022
dc.authorwosidsogutlu, fatma/ABE-2054-2020
dc.contributor.authorBiray Avcı, Çığır
dc.contributor.authorSoğutlu, Fatma
dc.contributor.authorÖzateş, Neslihan Pınar
dc.contributor.authorShademan, Behrouz
dc.contributor.authorGündüz, Cumhur
dc.date.accessioned2024-08-25T18:51:08Z
dc.date.available2024-08-25T18:51:08Z
dc.date.issued2023
dc.departmentEge Üniversitesien_US
dc.description.abstractPurpose: The phosphatidylinositol 3-kinase/AKT/mammalian target of rapamycin (PI3K/AKT/ mTOR) pathway is a complex intracellular metabolic pathway that leads to cell growth and tumor proliferation and plays a key role in drug resistance in breast cancer. Therefore, the anti-cancer effects of oleanolic acid (OA), maslinic acid (MA), and their combination were investigated to improve the performance of the treatment strategy. Methods: We investigated the effect of OA and MA on cell viability using the WST-1 method. The synergistic effect of the combination was analyzed by isobologram analysis. In addition, the effects of the two compounds, individually and in combination, on apoptosis, autophagy, and the cell cycle were investigated in MCF7 cells. In addition, changes in the expression of PI3K/AKT/mTOR genes involved in apoptosis, cell cycle and metabolism were determined by quantitative RT-PCR. Results: MA, OA, and a combination of both caused G0/G1 arrest. Apoptosis also increased in all treated groups. The autophagosomal LC3-II formation was induced 1.74-fold in the MA -treated group and 3.25-fold in the MA-OA-treated group. The combination treatment resulted in increased expression of genes such as GSK3B, PTEN, CDKN1B and FOXO3 and decreased expression of IGF1, PRKCB and AKT3 genes. Conclusion: The results showed that the combination of these two substances showed the highest synergistic effect at the lowest dose and using MA-OA caused cancer cells to undergo apoptosis. The use of combination drugs may reduce the resistance of cancer cells to treatment.en_US
dc.identifier.doi10.34172/apb.2023.057
dc.identifier.endpage620en_US
dc.identifier.issn2228-5881
dc.identifier.issn2251-7308
dc.identifier.issue3en_US
dc.identifier.pmid37646060en_US
dc.identifier.scopus2-s2.0-85166757871en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage611en_US
dc.identifier.urihttps://doi.org/10.34172/apb.2023.057
dc.identifier.urihttps://hdl.handle.net/11454/102486
dc.identifier.volume13en_US
dc.identifier.wosWOS:001044858800021en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTabriz Univ Medical Sciences & Health Servicesen_US
dc.relation.ispartofAdvanced Pharmaceutical Bulletinen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240825_Gen_US
dc.subjectBreast cancer mTOR signalen_US
dc.subjectpathway Therapeutic resistanceen_US
dc.subjectOleanolic acid Maslinic aciden_US
dc.subjectCell-Cycleen_US
dc.subjectApoptosisen_US
dc.subjectAutophagyen_US
dc.subjectSurvivalen_US
dc.subjectPathwayen_US
dc.subjectGrowthen_US
dc.subjectAkten_US
dc.titleEnhanced Anti-cancer Potency Using a Combination of Oleanolic Acid and Maslinic Acid to Control Treatment Resistance in Breast Canceren_US
dc.typeArticleen_US

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