Amperometric biosensors for detection of sugars based on the electrical wiring of different pyranose oxidases and pyranose dehydrogenases with osmium redox polymer on graphite electrodes

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dc.contributor.authorTasca, Federico
dc.contributor.authorTimur, Suna
dc.contributor.authorLudwig, Roland
dc.contributor.authorHaltrich, Dietmar
dc.contributor.authorVolc, Jindrich
dc.contributor.authorAntiochia, Riccarda
dc.contributor.authorGorton, Lo
dc.date.accessioned2019-10-27T19:37:31Z
dc.date.available2019-10-27T19:37:31Z
dc.date.issued2007
dc.departmentEge Üniversitesien_US
dc.description11th International Conference on Electroanalysis of the European-Society-for-Electroanalytical-Chemistry -- JUN 11-15, 2006 -- Univ Bordeaux, Natl Engn Sch Chem & Phys, Bordeaux, FRANCEen_US
dc.description.abstractElectrical wiring of different types of pyranose oxidase (P20) (fungal wild type, recombinant wild type with a hexahistidine tag, mutant form E542K with a hexa-histidine tag) from Trametes multicolor, and recombinant P20 from Coriolus sp. overexpressed in Escherichia coli as well as of pyranose dehydrogenase (PDH) from Agaricus meleagris and Agaricus xanthoderma with an osmium redox polymer (poly(1-vinylimidazole)(12)-[Os(4,4'-dimethyl-2,2'-dipyridyl)(2)Cl2+/+) on graphite electrodes was carried out. After optimization studies using glucose as substrate, the biosensors, which showed the best characteristics in terms of linear range, detection limit and sensitivity were selected, viz. wild type P20 from T multicolor and PDH from A. meleagris. These two enzymes were used and investigated for their selectivity for a number of different sugars.en_US
dc.description.sponsorshipEuropean Soc Electroanalyt Chemen_US
dc.identifier.doi10.1002/elan.200603740
dc.identifier.endpage302en_US
dc.identifier.issn1040-0397
dc.identifier.issn1521-4109
dc.identifier.issn1040-0397en_US
dc.identifier.issn1521-4109en_US
dc.identifier.issue02.Maren_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage294en_US
dc.identifier.urihttps://doi.org/10.1002/elan.200603740
dc.identifier.urihttps://hdl.handle.net/11454/39984
dc.identifier.volume19en_US
dc.identifier.wosWOS:000244220700025en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofElectroanalysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectnonselective sugar oxidizing enzymeen_US
dc.subjectflow injection analysisen_US
dc.subjectbiofuel cell anodeen_US
dc.titleAmperometric biosensors for detection of sugars based on the electrical wiring of different pyranose oxidases and pyranose dehydrogenases with osmium redox polymer on graphite electrodesen_US
dc.typeArticleen_US

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