Amperometric biosensors for detection of sugars based on the electrical wiring of different pyranose oxidases and pyranose dehydrogenases with osmium redox polymer on graphite electrodes
Küçük Resim Yok
Tarih
2007
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-V C H Verlag Gmbh
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Electrical wiring of different types of pyranose oxidase (P20) (fungal wild type, recombinant wild type with a hexahistidine tag, mutant form E542K with a hexa-histidine tag) from Trametes multicolor, and recombinant P20 from Coriolus sp. overexpressed in Escherichia coli as well as of pyranose dehydrogenase (PDH) from Agaricus meleagris and Agaricus xanthoderma with an osmium redox polymer (poly(1-vinylimidazole)(12)-[Os(4,4'-dimethyl-2,2'-dipyridyl)(2)Cl2+/+) on graphite electrodes was carried out. After optimization studies using glucose as substrate, the biosensors, which showed the best characteristics in terms of linear range, detection limit and sensitivity were selected, viz. wild type P20 from T multicolor and PDH from A. meleagris. These two enzymes were used and investigated for their selectivity for a number of different sugars.
Açıklama
11th International Conference on Electroanalysis of the European-Society-for-Electroanalytical-Chemistry -- JUN 11-15, 2006 -- Univ Bordeaux, Natl Engn Sch Chem & Phys, Bordeaux, FRANCE
Anahtar Kelimeler
nonselective sugar oxidizing enzyme, flow injection analysis, biofuel cell anode
Kaynak
Electroanalysis
WoS Q Değeri
Q1
Scopus Q Değeri
Q2
Cilt
19
Sayı
02.Mar
