Identification of Potential Serodiagnostic and Subunit Vaccine Antigens by Antibody Profiling of Toxoplasmosis Cases in Turkey

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dc.contributor.authorLiang, Li
dc.contributor.authorDoskaya, Mert
dc.contributor.authorJuarez, Silvia
dc.contributor.authorCaner, Ayse
dc.contributor.authorJasinskas, Algis
dc.contributor.authorTan, Xiaolin
dc.contributor.authorHajagos, Bettina E.
dc.contributor.authorBradley, Peter J.
dc.contributor.authorKorkmaz, Metin
dc.contributor.authorGuruz, Yuksel
dc.contributor.authorFelgner, Philip L.
dc.contributor.authorDavies, D. Huw
dc.date.accessioned2019-10-27T21:38:00Z
dc.date.available2019-10-27T21:38:00Z
dc.date.issued2011
dc.departmentEge Üniversitesien_US
dc.description.abstractToxoplasmosis, caused by infection of the protozoan parasite Toxoplasma gondii, is associated with mild disease in healthy individuals, whereas individuals with depressed immunity may develop encephalitis, neurologic disorders, and other organ diseases. Women who develop acute toxoplasmosis during pregnancy are at risk of transmitting the infection to the fetus, which may lead to fetal damage. A diagnosis is usually confirmed by measuring IgG, or IgM where it is important to determine the onset of infection. A negative IgM result essentially excludes acute infection, whereas a positive IgM test is largely uninterpretable because IgM can persist for up to 18 months after infection. To identify antigens for improved diagnosis of acute infection, we probed protein microarrays displaying the polypeptide products of 1357 Toxoplasma exons with well-characterized sera from Turkey. The sera were classified according to conventional assays into (1) seronegative individuals with no history of T. gondii infection; (2) acute infections defined by clinical symptoms, high IgM titers, and low avidity IgG; (3) chronic/convalescent cases with high avidity IgG but persisting IgM; (iv) true chronic infections, defined by high avidity IgG and no IgM. We have identified 38 IgG target antigens and 108 IgM target antigens that can discriminate infected patients from healthy controls, one or more of which could form the basis of a 'tier-1' test to determine current or previous exposure. Of these, three IgG antigens and five IgM antigens have the potential to discriminate chronic/IgM persisting or true chronics from recent acutely infected patients (a 'tier-2' test). Our analysis of the antigens revealed several enriched features relative to the whole proteome, which include transmembrane domains, signal peptides, or predicted localization at the outer membrane. This is the first protein microarray survey of the antibody response to T. gondii, and will help in the development of improved serodiagnostics and vaccines. Molecular & Cellular Proteomics 10: 10.1074/mcp.M110.006916, 1-17, 2011.en_US
dc.description.sponsorshipNational Institute of Allergy and Infectious DiseasesUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Allergy & Infectious Diseases (NIAID) [AI058365, AI064616, AI07323]; Scientific Research Projects Branch Directorate of Ege University Chancellery, TurkeyEge University [2007TIP015]en_US
dc.description.sponsorshipThis work was supported by an R44 grant (AI058365) from the National Institute of Allergy and Infectious Diseases (D. H. D.), an RO1 grant (AI064616) to P.J.B. and a Microbial Pathogenesis Training Grant T32-AI07323 to B. E. H. The research was also supported by the Scientific Research Projects Branch Directorate of Ege University Chancellery, Turkey (grant 2007TIP015) to Y.G.en_US
dc.identifier.doi10.1074/mcp.M110.006916en_US
dc.identifier.issn1535-9476
dc.identifier.issn1535-9484
dc.identifier.issue7en_US
dc.identifier.pmid21512035en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1074/mcp.M110.006916
dc.identifier.urihttps://hdl.handle.net/11454/46297
dc.identifier.volume10en_US
dc.identifier.wosWOS:000292541500006en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmer Soc Biochemistry Molecular Biology Incen_US
dc.relation.ispartofMolecular & Cellular Proteomicsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleIdentification of Potential Serodiagnostic and Subunit Vaccine Antigens by Antibody Profiling of Toxoplasmosis Cases in Turkeyen_US
dc.typeArticleen_US

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