Graphene-Oxide and Ionic Liquid Modified Electrodes for Electrochemical Sensing of Breast Cancer 1 Gene
Küçük Resim Yok
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Mdpi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Graphene-oxide and ionic liquid composite-modified pencil graphite electrodes (GO-IL-PGEs) were developed and used as a sensing platform for breast cancer 1 (BRCA1) gene detection. The characterization of GO-IL modified electrodes was executed by scanning electron microscopy (SEM), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The nucleic-acid hybridization was monitored by a differential pulse voltammetry (DPV) technique by directly measuring the guanine oxidation signal without using any indicator. The effects of the IL concentration, the probe concentration, and the hybridization time were optimized to the biosensor response. The limit of detection (LOD) was calculated in the concentration range of 2-10 mu g/mL for the BRCA1 gene and found to be 1.48 mu g/mL. The sensitivity of the sensor was calculated as 1.49 mu A mL/mu g cm(2). The developed biosensor can effectively discriminate the complementary target sequence in comparison to a three-base-mismatched sequence or the non-complementary one.
Açıklama
Anahtar Kelimeler
graphene oxide, ionic liquid, electrochemical nucleic acid sensor, breast cancer 1 gene, DNA hybridization, Label-Free, Sensitive Detection, Graphite-Electrodes, Biosensor, Sensor, Nanomaterial, Platform
Kaynak
Biosensors-Basel
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
12
Sayı
2
