Polythiophene-g-poly(ethylene glycol) with Lateral Amino Groups as a Novel Matrix for Biosensor Construction

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dc.contributor.authorAkbulut H.
dc.contributor.authorBozokalfa G.
dc.contributor.authorAsker D.N.
dc.contributor.authorDemir B.
dc.contributor.authorGuler E.
dc.contributor.authorOdaci Demirkol D.
dc.contributor.authorTimur S.
dc.contributor.authorYagci Y.
dc.date.accessioned2019-10-26T21:21:16Z
dc.date.available2019-10-26T21:21:16Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractIn the ever-expanding field of conducting polymer research, functionalized graft hybrid copolymers have gained considerable interest in the biomedical engineering and biosensing applications, particularly. In the present work, a new biosensor based on conducting graft copolymer for the detection of phenolic compounds was developed. Thereby, a robust and novel material, namely "polythiophene-g-poly(ethylene glycol) with lateral amino groups" (PT-NH<inf>2</inf>-g-PEG) hybrid conducting polymer was synthesized via Suzuki condensation polymerization and characterized with 1H NMR analysis, UV-vis spectroscopy, gel permeation chromatography (GPC) and fluorescence spectroscopy. PT-NH<inf>2</inf>-g-PEG architecture was then applied as an immobilization matrix to accomplish extended biosensing function. In a typical process, Laccase was utilized as a model enzyme for the detection of phenolic compounds. Detailed surface characterization of PT-NH<inf>2</inf>-g-PEG/Lac was performed by cyclic voltammetry, electrochemical impedance spectroscopy, atomic force microscopy, fluorescence microscopy and scanning electron microscopy measurements. Optimum pH and polymer amount were found to be pH 6.5 and 0.5 mg polymer, respectively, with the linear range of 0.0025-0.05 mM and 132.45 µA/mM sensitivity. The kinetic parameters of PT-NH<inf>2</inf>-g-PEG/Lac are 0.026 mM for K<inf>m</inf>app and 7.38 µA for I<inf>max</inf>, respectively. Furthermore, the PT-NH<inf>2</inf>-g-PEG/Lac biofilm was retained 82% of its activity for 12 days indicating excellent recovery as tested with artificial wastewater. © 2015 American Chemical Society.en_US
dc.identifier.doi10.1021/acsami.5b04967en_US
dc.identifier.endpage20622en_US
dc.identifier.issn1944-8244
dc.identifier.issue37en_US
dc.identifier.pmid26323569en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage20612en_US
dc.identifier.urihttps://doi.org/10.1021/acsami.5b04967
dc.identifier.urihttps://hdl.handle.net/11454/16855
dc.identifier.volume7en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.ispartofACS Applied Materials and Interfacesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbiomolecule immobilizationen_US
dc.subjectbiosensoren_US
dc.subjectconducting polymersen_US
dc.subjectgraft copolymeren_US
dc.subjectpoly(ethylene glycol)en_US
dc.subjectpolythiopheneen_US
dc.subjectsurface modificationen_US
dc.titlePolythiophene-g-poly(ethylene glycol) with Lateral Amino Groups as a Novel Matrix for Biosensor Constructionen_US
dc.typeArticleen_US

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