In Vitro Human Blood-Brain Barrier Model for Drug Permeability Testing
Küçük Resim Yok
Tarih
2021
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Humana Press Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Blood-brain barrier (BBB), although very important for protection of brain from major neurotoxins, negatively affects the treatment of central nervous system diseases by limiting the passage of neuropharmaceuticals from blood to the brain. Thus, researchers have to investigate the passage of the produced drug molecules through the BBB before they are introduced to the market. Although these experiments have been traditionally performed on experimental animals, drug permeability tests are now carried out mostly by in vitro BBB models due to ethical problems, differences between species, and expensive and troublesome in vivo test procedures. In this method, we explain how to model and characterize a realistic in vitro BBB model using human derived cells and perform a drug permeability test using this model.
Açıklama
Anahtar Kelimeler
Astrocytes, Blood-brain barrier, Brain microvascular endothelial cells, Brain microvascular pericytes, Modeling barrier tissues, Permeability, Tight junctions, Tight Junction, Transporters, Pericytes, Delivery
Kaynak
Permeability Barrier, 2 Edition: Methods and Protocols
WoS Q Değeri
N/A
Scopus Q Değeri
Q3
Cilt
2367
