Immunohistochemical profile of transforming growth factor-beta 1 and basic fibroblast growth factor in sciatic nerve anastomosis following pinealectomy and exogenous melatonin administration in rats

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dc.contributor.authorTurgut, Mehmet
dc.contributor.authorOektem, Guelperi
dc.contributor.authorUysal, Ayseguel
dc.contributor.authorYurtseven, Mine Ertem
dc.date.accessioned2019-10-27T19:17:39Z
dc.date.available2019-10-27T19:17:39Z
dc.date.issued2006
dc.departmentEge Üniversitesien_US
dc.description.abstractCollagen sear formation at the cut end of a peripheral nerve, an important problem in clinical practice for neurosurgeons, obstructs sprouting of axons into appropriate distal fascicles, and thereby limits the regeneration process. Researchers have attempted to control collagen accumulation and neuroma formation with various physical and chemical methods, but with limited functional success. Recently, it has been demonstrated that transforming growth factor (TGF)-beta and basic fibroblast growth factor (bFGF) play an important role in collagen production by fibroblasts and in Schwann cell activity. In our study, rats were divided into a control group, a melatonin-treated group, a surgical pinealectomy group, and a group treated with melatonin following pinealectomy. They then underwent a surgical sciatic nerve transection and primary suture anastomosis. At 2 months after anastomosis, the animals were sacrificed and unilateral sciatic nerve specimens, including the anastomotic region, were removed and processed for immunohistochemical study from two animals in each group. For each antibody, immunoreactivity was assessed using a semiquantitative scoring system. Strong TGF-beta 1 and/ or bFGF expression was observed in the epineurium of animals that underwent pinealectomy, but no or weak staining was observed in animals in the control and melatonin treatment groups. Based on these data, we suggest that both TGF-beta 1 and bFGF have important roles in control of collagen accumulation and neuroma formation at the anastomotic site, and that the pineal neurohormone melatonin has a beneficial effect on nerve regeneration. (C) 2006 Elsevier Ltd. All rights reserved.en_US
dc.identifier.doi10.1016/j.jocn.2005.07.019en_US
dc.identifier.endpage758en_US
dc.identifier.issn0967-5868
dc.identifier.issue7en_US
dc.identifier.pmid16831553en_US
dc.identifier.startpage753en_US
dc.identifier.urihttps://doi.org/10.1016/j.jocn.2005.07.019
dc.identifier.urihttps://hdl.handle.net/11454/38438
dc.identifier.volume13en_US
dc.identifier.wosWOS:000241592300009en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherChurchill Livingstoneen_US
dc.relation.ispartofJournal of Clinical Neuroscienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbasic fibroblast growth factoren_US
dc.subjectcollagenen_US
dc.subjectpinealectomyen_US
dc.subjectmelatoninen_US
dc.subjectregenerationen_US
dc.subjecttransforming growth factor-beta 1en_US
dc.subjectsciatic nerveen_US
dc.titleImmunohistochemical profile of transforming growth factor-beta 1 and basic fibroblast growth factor in sciatic nerve anastomosis following pinealectomy and exogenous melatonin administration in ratsen_US
dc.typeArticleen_US

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