Analysis of long non-coding RNA (lncRNA) expression in hepatitis B patients

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dc.contributor.authorSusluer, Sunde Yilmaz
dc.contributor.authorKayabasi, Cagla
dc.contributor.authorYelken, Besra Ozmen
dc.contributor.authorAsik, Aycan
dc.contributor.authorCelik, Didem
dc.contributor.authorOkcanoglu, Tugce Balci
dc.contributor.authorSenger, Suheyla Serin
dc.contributor.authorAvci, Cigir Biray
dc.contributor.authorKose, Sukran
dc.contributor.authorGunduz, Cumhur
dc.date.accessioned2019-10-27T10:45:55Z
dc.date.available2019-10-27T10:45:55Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractLong non-coding RNAs (lncRNAs) have been implicated in numerous biological processes, including epigenetic regulation, cell-cycle control, and transcriptional/translational regulation of gene expression. Differential expression of lncRNAs and disruption of the regulatory processes are recognized as critical steps in cancer development. The role of lncRNAs in hepatitis B virus (HBV) infection is not well understood. Here we analyzed the expression of 135 lncRNAs in plasma samples of 82 HBV patients (classified as chronic patients, inactive carriers, or resolved patients) at diagnosis and at 12 months of treatment in relation to control group (81 healthy volunteers). We also investigated the effect of small interfering RNA (siRNA)-mediated silencing of lincRNA-SFMBT2 on HBV-positive human liver cancer cell line. lncRNA expression was analyzed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Chemically synthesized siRNAs were transfected into the cell lines using Lipofectamine 2000 Reagent (Thermo Fisher Scientific). HBV DNA and HBsAg and HBeAg were detected in transfected cultures by real-time PCR and ELISA, respectively, using commercial kits. We observed changes in lncRNA expression in all three HBV groups, compared to control group. Most notably, the expression of anti-NOS2A, lincRNA-SFMBT2, and Zfhx2as was significantly increased and expression of Y5 lncRNA was decreased in chronic HBV patients. A decreased Y5 expression and increased lincRNA-SFMBT2 expression were observed in inactive HBsAg carriers. The expression of HOTTIP, MEG9, and PCAT-32 was increased in resolved HBV patients, and no significant change in the expression of Y5 was observed, compared to control group. siRNA-mediated inhibition of lincRNA-SFMBT2 decreased the level of HBV DNA in human liver cancer cells. Further research is needed to confirm the prognostic as well as therapeutic role of these lncRNAs in HBV patients.en_US
dc.description.sponsorshipTUBITAK (the Scientific and Technological Research Council of Turkey) grantTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [215S225]en_US
dc.description.sponsorshipThis work was supported by TUBITAK (the Scientific and Technological Research Council of Turkey) grant (Project No: 215S225).en_US
dc.identifier.doi10.17305/bjbms.2018.2800en_US
dc.identifier.endpage161en_US
dc.identifier.issn1512-8601
dc.identifier.issn1840-4812
dc.identifier.issue2en_US
dc.identifier.pmid29669510en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage150en_US
dc.identifier.urihttps://doi.org/10.17305/bjbms.2018.2800
dc.identifier.urihttps://hdl.handle.net/11454/31223
dc.identifier.volume18en_US
dc.identifier.wosWOS:000433285000006en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAssoc Basic Medical Sci Federation Bosnia & Herzegovina Sarajevoen_US
dc.relation.ispartofBosnian Journal of Basic Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectHBVen_US
dc.subjectlncRNAen_US
dc.subjectchronic hepatitis Ben_US
dc.subjectinactive HBsAg carrieren_US
dc.subjectresolved hepatitis Ben_US
dc.titleAnalysis of long non-coding RNA (lncRNA) expression in hepatitis B patientsen_US
dc.typeArticleen_US

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